an example of hypothesis testing

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Statistics By Jim

Making statistics intuitive

Statistical Hypothesis Testing Overview

By Jim Frost 59 Comments

In this blog post, I explain why you need to use statistical hypothesis testing and help you navigate the essential terminology. Hypothesis testing is a crucial procedure to perform when you want to make inferences about a population using a random sample. These inferences include estimating population properties such as the mean, differences between means, proportions, and the relationships between variables.

This post provides an overview of statistical hypothesis testing. If you need to perform hypothesis tests, consider getting my book, Hypothesis Testing: An Intuitive Guide .

Why You Should Perform Statistical Hypothesis Testing

Hypothesis testing is a form of inferential statistics that allows us to draw conclusions about an entire population based on a representative sample. You gain tremendous benefits by working with a sample. In most cases, it is simply impossible to observe the entire population to understand its properties. The only alternative is to collect a random sample and then use statistics to analyze it.

While samples are much more practical and less expensive to work with, there are trade-offs. When you estimate the properties of a population from a sample, the sample statistics are unlikely to equal the actual population value exactly.  For instance, your sample mean is unlikely to equal the population mean. The difference between the sample statistic and the population value is the sample error.

Differences that researchers observe in samples might be due to sampling error rather than representing a true effect at the population level. If sampling error causes the observed difference, the next time someone performs the same experiment the results might be different. Hypothesis testing incorporates estimates of the sampling error to help you make the correct decision. Learn more about Sampling Error .

For example, if you are studying the proportion of defects produced by two manufacturing methods, any difference you observe between the two sample proportions might be sample error rather than a true difference. If the difference does not exist at the population level, you won’t obtain the benefits that you expect based on the sample statistics. That can be a costly mistake!

Let’s cover some basic hypothesis testing terms that you need to know.

Background information : Difference between Descriptive and Inferential Statistics and Populations, Parameters, and Samples in Inferential Statistics

Hypothesis Testing

Hypothesis testing is a statistical analysis that uses sample data to assess two mutually exclusive theories about the properties of a population. Statisticians call these theories the null hypothesis and the alternative hypothesis. A hypothesis test assesses your sample statistic and factors in an estimate of the sample error to determine which hypothesis the data support.

When you can reject the null hypothesis, the results are statistically significant, and your data support the theory that an effect exists at the population level.

The effect is the difference between the population value and the null hypothesis value. The effect is also known as population effect or the difference. For example, the mean difference between the health outcome for a treatment group and a control group is the effect.

Typically, you do not know the size of the actual effect. However, you can use a hypothesis test to help you determine whether an effect exists and to estimate its size. Hypothesis tests convert your sample effect into a test statistic, which it evaluates for statistical significance. Learn more about Test Statistics .

An effect can be statistically significant, but that doesn’t necessarily indicate that it is important in a real-world, practical sense. For more information, read my post about Statistical vs. Practical Significance .

Null Hypothesis

The null hypothesis is one of two mutually exclusive theories about the properties of the population in hypothesis testing. Typically, the null hypothesis states that there is no effect (i.e., the effect size equals zero). The null is often signified by H 0 .

In all hypothesis testing, the researchers are testing an effect of some sort. The effect can be the effectiveness of a new vaccination, the durability of a new product, the proportion of defect in a manufacturing process, and so on. There is some benefit or difference that the researchers hope to identify.

However, it’s possible that there is no effect or no difference between the experimental groups. In statistics, we call this lack of an effect the null hypothesis. Therefore, if you can reject the null, you can favor the alternative hypothesis, which states that the effect exists (doesn’t equal zero) at the population level.

You can think of the null as the default theory that requires sufficiently strong evidence against in order to reject it.

For example, in a 2-sample t-test, the null often states that the difference between the two means equals zero.

When you can reject the null hypothesis, your results are statistically significant. Learn more about Statistical Significance: Definition & Meaning .

Related post : Understanding the Null Hypothesis in More Detail

Alternative Hypothesis

The alternative hypothesis is the other theory about the properties of the population in hypothesis testing. Typically, the alternative hypothesis states that a population parameter does not equal the null hypothesis value. In other words, there is a non-zero effect. If your sample contains sufficient evidence, you can reject the null and favor the alternative hypothesis. The alternative is often identified with H 1 or H A .

For example, in a 2-sample t-test, the alternative often states that the difference between the two means does not equal zero.

You can specify either a one- or two-tailed alternative hypothesis:

If you perform a two-tailed hypothesis test, the alternative states that the population parameter does not equal the null value. For example, when the alternative hypothesis is H A : μ ≠ 0, the test can detect differences both greater than and less than the null value.

A one-tailed alternative has more power to detect an effect but it can test for a difference in only one direction. For example, H A : μ > 0 can only test for differences that are greater than zero.

Related posts : Understanding T-tests and One-Tailed and Two-Tailed Hypothesis Tests Explained

P-values are the probability that you would obtain the effect observed in your sample, or larger, if the null hypothesis is correct. In simpler terms, p-values tell you how strongly your sample data contradict the null. Lower p-values represent stronger evidence against the null. You use P-values in conjunction with the significance level to determine whether your data favor the null or alternative hypothesis.

Related post : Interpreting P-values Correctly

Significance Level (Alpha)

For instance, a significance level of 0.05 signifies a 5% risk of deciding that an effect exists when it does not exist.

Use p-values and significance levels together to help you determine which hypothesis the data support. If the p-value is less than your significance level, you can reject the null and conclude that the effect is statistically significant. In other words, the evidence in your sample is strong enough to be able to reject the null hypothesis at the population level.

Related posts : Graphical Approach to Significance Levels and P-values and Conceptual Approach to Understanding Significance Levels

Types of Errors in Hypothesis Testing

Statistical hypothesis tests are not 100% accurate because they use a random sample to draw conclusions about entire populations. There are two types of errors related to drawing an incorrect conclusion.

• False positives: You reject a null that is true. Statisticians call this a Type I error . The Type I error rate equals your significance level or alpha (α).
• False negatives: You fail to reject a null that is false. Statisticians call this a Type II error. Generally, you do not know the Type II error rate. However, it is a larger risk when you have a small sample size , noisy data, or a small effect size. The type II error rate is also known as beta (β).

Statistical power is the probability that a hypothesis test correctly infers that a sample effect exists in the population. In other words, the test correctly rejects a false null hypothesis. Consequently, power is inversely related to a Type II error. Power = 1 – β. Learn more about Power in Statistics .

Related posts : Types of Errors in Hypothesis Testing and Estimating a Good Sample Size for Your Study Using Power Analysis

Which Type of Hypothesis Test is Right for You?

There are many different types of procedures you can use. The correct choice depends on your research goals and the data you collect. Do you need to understand the mean or the differences between means? Or, perhaps you need to assess proportions. You can even use hypothesis testing to determine whether the relationships between variables are statistically significant.

To choose the proper statistical procedure, you’ll need to assess your study objectives and collect the correct type of data . This background research is necessary before you begin a study.

Related Post : Hypothesis Tests for Continuous, Binary, and Count Data

Statistical tests are crucial when you want to use sample data to make conclusions about a population because these tests account for sample error. Using significance levels and p-values to determine when to reject the null hypothesis improves the probability that you will draw the correct conclusion.

To see an alternative approach to these traditional hypothesis testing methods, learn about bootstrapping in statistics !

If you want to see examples of hypothesis testing in action, I recommend the following posts that I have written:

• How Effective Are Flu Shots? This example shows how you can use statistics to test proportions.
• Fatality Rates in Star Trek . This example shows how to use hypothesis testing with categorical data.
• Busting Myths About the Battle of the Sexes . A fun example based on a Mythbusters episode that assess continuous data using several different tests.
• Are Yawns Contagious? Another fun example inspired by a Mythbusters episode.

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Reader Interactions

January 14, 2024 at 8:43 am

Hello professor Jim, how are you doing! Pls. What are the properties of a population and their examples? Thanks for your time and understanding.

January 14, 2024 at 12:57 pm

Please read my post about Populations vs. Samples for more information and examples.

Also, please note there is a search bar in the upper-right margin of my website. Use that to search for topics.

July 5, 2023 at 7:05 am

Hello, I have a question as I read your post. You say in p-values section

“P-values are the probability that you would obtain the effect observed in your sample, or larger, if the null hypothesis is correct. In simpler terms, p-values tell you how strongly your sample data contradict the null. Lower p-values represent stronger evidence against the null.”

But according to your definition of effect, the null states that an effect does not exist, correct? So what I assume you want to say is that “P-values are the probability that you would obtain the effect observed in your sample, or larger, if the null hypothesis is **incorrect**.”

July 6, 2023 at 5:18 am

Hi Shrinivas,

The correct definition of p-value is that it is a probability that exists in the context of a true null hypothesis. So, the quotation is correct in stating “if the null hypothesis is correct.”

Essentially, the p-value tells you the likelihood of your observed results (or more extreme) if the null hypothesis is true. It gives you an idea of whether your results are surprising or unusual if there is no effect.

Hence, with sufficiently low p-values, you reject the null hypothesis because it’s telling you that your sample results were unlikely to have occurred if there was no effect in the population.

I hope that helps make it more clear. If not, let me know I’ll attempt to clarify!

May 8, 2023 at 12:47 am

Thanks a lot Ny best regards

May 7, 2023 at 11:15 pm

Hi Jim Can you tell me something about size effect? Thanks

May 8, 2023 at 12:29 am

Here’s a post that I’ve written about Effect Sizes that will hopefully tell you what you need to know. Please read that. Then, if you have any more specific questions about effect sizes, please post them there. Thanks!

January 7, 2023 at 4:19 pm

Hi Jim, I have only read two pages so far but I am really amazed because in few paragraphs you made me clearly understand the concepts of months of courses I received in biostatistics! Thanks so much for this work you have done it helps a lot!

January 10, 2023 at 3:25 pm

Thanks so much!

June 17, 2021 at 1:45 pm

Can you help in the following question: Rocinante36 is priced at ₹7 lakh and has been designed to deliver a mileage of 22 km/litre and a top speed of 140 km/hr. Formulate the null and alternative hypotheses for mileage and top speed to check whether the new models are performing as per the desired design specifications.

April 19, 2021 at 1:51 pm

Its indeed great to read your work statistics.

I have a doubt regarding the one sample t-test. So as per your book on hypothesis testing with reference to page no 45, you have mentioned the difference between “the sample mean and the hypothesised mean is statistically significant”. So as per my understanding it should be quoted like “the difference between the population mean and the hypothesised mean is statistically significant”. The catch here is the hypothesised mean represents the sample mean.

Please help me understand this.

Regards Rajat

April 19, 2021 at 3:46 pm

Thanks for buying my book. I’m so glad it’s been helpful!

The test is performed on the sample but the results apply to the population. Hence, if the difference between the sample mean (observed in your study) and the hypothesized mean is statistically significant, that suggests that population does not equal the hypothesized mean.

For one sample tests, the hypothesized mean is not the sample mean. It is a mean that you want to use for the test value. It usually represents a value that is important to your research. In other words, it’s a value that you pick for some theoretical/practical reasons. You pick it because you want to determine whether the population mean is different from that particular value.

I hope that helps!

November 5, 2020 at 6:24 am

Jim, you are such a magnificent statistician/economist/econometrician/data scientist etc whatever profession. Your work inspires and simplifies the lives of so many researchers around the world. I truly admire you and your work. I will buy a copy of each book you have on statistics or econometrics. Keep doing the good work. Remain ever blessed

November 6, 2020 at 9:47 pm

Hi Renatus,

Thanks so much for you very kind comments. You made my day!! I’m so glad that my website has been helpful. And, thanks so much for supporting my books! 🙂

November 2, 2020 at 9:32 pm

Hi Jim, I hope you are aware of 2019 American Statistical Association’s official statement on Statistical Significance: https://www.tandfonline.com/doi/full/10.1080/00031305.2019.1583913 In case you do not bother reading the full article, may I quote you the core message here: “We conclude, based on our review of the articles in this special issue and the broader literature, that it is time to stop using the term “statistically significant” entirely. Nor should variants such as “significantly different,” “p < 0.05,” and “nonsignificant” survive, whether expressed in words, by asterisks in a table, or in some other way."

With best wishes,

November 3, 2020 at 2:09 am

I’m definitely aware of the debate surrounding how to use p-values most effectively. However, I need to correct you on one point. The link you provide is NOT a statement by the American Statistical Association. It is an editorial by several authors.

There is considerable debate over this issue. There are problems with p-values. However, as the authors state themselves, much of the problem is over people’s mindsets about how to use p-values and their incorrect interpretations about what statistical significance does and does not mean.

If you were to read my website more thoroughly, you’d be aware that I share many of their concerns and I address them in multiple posts. One of the authors’ key points is the need to be thoughtful and conduct thoughtful research and analysis. I emphasize this aspect in multiple posts on this topic. I’ll ask you to read the following three because they all address some of the authors’ concerns and suggestions. But you might run across others to read as well.

Five Tips for Using P-values to Avoid Being Misled How to Interpret P-values Correctly P-values and the Reproducibility of Experimental Results

September 24, 2020 at 11:52 pm

HI Jim, i just want you to know that you made explanation for Statistics so simple! I should say lesser and fewer words that reduce the complexity. All the best! 🙂

September 25, 2020 at 1:03 am

Thanks, Rene! Your kind words mean a lot to me! I’m so glad it has been helpful!

September 23, 2020 at 2:21 am

Honestly, I never understood stats during my entire M.Ed course and was another nightmare for me. But how easily you have explained each concept, I have understood stats way beyond my imagination. Thank you so much for helping ignorant research scholars like us. Looking forward to get hardcopy of your book. Kindly tell is it available through flipkart?

September 24, 2020 at 11:14 pm

I’m so happy to hear that my website has been helpful!

I checked on flipkart and it appears like my books are not available there. I’m never exactly sure where they’re available due to the vagaries of different distribution channels. They are available on Amazon in India.

Introduction to Statistics: An Intuitive Guide (Amazon IN) Hypothesis Testing: An Intuitive Guide (Amazon IN)

July 26, 2020 at 11:57 am

Dear Jim I am a teacher from India . I don’t have any background in statistics, and still I should tell that in a single read I can follow your explanations . I take my entire biostatistics class for botany graduates with your explanations. Thanks a lot. May I know how I can avail your books in India

July 28, 2020 at 12:31 am

Right now my books are only available as ebooks from my website. However, soon I’ll have some exciting news about other ways to obtain it. Stay tuned! I’ll announce it on my email list. If you’re not already on it, you can sign up using the form that is in the right margin of my website.

June 22, 2020 at 2:02 pm

Also can you please let me if this book covers topics like EDA and principal component analysis?

June 22, 2020 at 2:07 pm

This book doesn’t cover principal components analysis. Although, I wouldn’t really classify that as a hypothesis test. In the future, I might write a multivariate analysis book that would cover this and others. But, that’s well down the road.

My Introduction to Statistics covers EDA. That’s the largely graphical look at your data that you often do prior to hypothesis testing. The Introduction book perfectly leads right into the Hypothesis Testing book.

June 22, 2020 at 1:45 pm

Thanks for the detailed explanation. It does clear my doubts. I saw that your book related to hypothesis testing has the topics that I am studying currently. I am looking forward to purchasing it.

Regards, Take Care

June 19, 2020 at 1:03 pm

For this particular article I did not understand a couple of statements and it would great if you could help: 1)”If sample error causes the observed difference, the next time someone performs the same experiment the results might be different.” 2)”If the difference does not exist at the population level, you won’t obtain the benefits that you expect based on the sample statistics.”

I discovered your articles by chance and now I keep coming back to read & understand statistical concepts. These articles are very informative & easy to digest. Thanks for the simplifying things.

June 20, 2020 at 9:53 pm

I’m so happy to hear that you’ve found my website to be helpful!

To answer your questions, keep in mind that a central tenant of inferential statistics is that the random sample that a study drew was only one of an infinite number of possible it could’ve drawn. Each random sample produces different results. Most results will cluster around the population value assuming they used good methodology. However, random sampling error always exists and makes it so that population estimates from a sample almost never exactly equal the correct population value.

So, imagine that we’re studying a medication and comparing the treatment and control groups. Suppose that the medicine is truly not effect and that the population difference between the treatment and control group is zero (i.e., no difference.) Despite the true difference being zero, most sample estimates will show some degree of either a positive or negative effect thanks to random sampling error. So, just because a study has an observed difference does not mean that a difference exists at the population level. So, on to your questions:

1. If the observed difference is just random error, then it makes sense that if you collected another random sample, the difference could change. It could change from negative to positive, positive to negative, more extreme, less extreme, etc. However, if the difference exists at the population level, most random samples drawn from the population will reflect that difference. If the medicine has an effect, most random samples will reflect that fact and not bounce around on both sides of zero as much.

2. This is closely related to the previous answer. If there is no difference at the population level, but say you approve the medicine because of the observed effects in a sample. Even though your random sample showed an effect (which was really random error), that effect doesn’t exist. So, when you start using it on a larger scale, people won’t benefit from the medicine. That’s why it’s important to separate out what is easily explained by random error versus what is not easily explained by it.

I think reading my post about how hypothesis tests work will help clarify this process. Also, in about 24 hours (as I write this), I’ll be releasing my new ebook about Hypothesis Testing!

May 29, 2020 at 5:23 am

Hi Jim, I really enjoy your blog. Can you please link me on your blog where you discuss about Subgroup analysis and how it is done? I need to use non parametric and parametric statistical methods for my work and also do subgroup analysis in order to identify potential groups of patients that may benefit more from using a treatment than other groups.

May 29, 2020 at 2:12 pm

Hi, I don’t have a specific article about subgroup analysis. However, subgroup analysis is just the dividing up of a larger sample into subgroups and then analyzing those subgroups separately. You can use the various analyses I write about on the subgroups.

Alternatively, you can include the subgroups in regression analysis as an indicator variable and include that variable as a main effect and an interaction effect to see how the relationships vary by subgroup without needing to subdivide your data. I write about that approach in my article about comparing regression lines . This approach is my preferred approach when possible.

April 19, 2020 at 7:58 am

sir is confidence interval is a part of estimation?

April 17, 2020 at 3:36 pm

Sir can u plz briefly explain alternatives of hypothesis testing? I m unable to find the answer

April 18, 2020 at 1:22 am

Assuming you want to draw conclusions about populations by using samples (i.e., inferential statistics ), you can use confidence intervals and bootstrap methods as alternatives to the traditional hypothesis testing methods.

March 9, 2020 at 10:01 pm

Hi JIm, could you please help with activities that can best teach concepts of hypothesis testing through simulation, Also, do you have any question set that would enhance students intuition why learning hypothesis testing as a topic in introductory statistics. Thanks.

March 5, 2020 at 3:48 pm

Hi Jim, I’m studying multiple hypothesis testing & was wondering if you had any material that would be relevant. I’m more trying to understand how testing multiple samples simultaneously affects your results & more on the Bonferroni Correction

March 5, 2020 at 4:05 pm

I write about multiple comparisons (aka post hoc tests) in the ANOVA context . I don’t talk about Bonferroni Corrections specifically but I cover related types of corrections. I’m not sure if that exactly addresses what you want to know but is probably the closest I have already written. I hope it helps!

January 14, 2020 at 9:03 pm

Thank you! Have a great day/evening.

January 13, 2020 at 7:10 pm

Any help would be greatly appreciated. What is the difference between The Hypothesis Test and The Statistical Test of Hypothesis?

January 14, 2020 at 11:02 am

They sound like the same thing to me. Unless this is specialized terminology for a particular field or the author was intending something specific, I’d guess they’re one and the same.

April 1, 2019 at 10:00 am

so these are the only two forms of Hypothesis used in statistical testing?

April 1, 2019 at 10:02 am

Are you referring to the null and alternative hypothesis? If so, yes, that’s those are the standard hypotheses in a statistical hypothesis test.

April 1, 2019 at 9:57 am

year very insightful post, thanks for the write up

October 27, 2018 at 11:09 pm

hi there, am upcoming statistician, out of all blogs that i have read, i have found this one more useful as long as my problem is concerned. thanks so much

October 27, 2018 at 11:14 pm

Hi Stano, you’re very welcome! Thanks for your kind words. They mean a lot! I’m happy to hear that my posts were able to help you. I’m sure you will be a fantastic statistician. Best of luck with your studies!

October 26, 2018 at 11:39 am

Dear Jim, thank you very much for your explanations! I have a question. Can I use t-test to compare two samples in case each of them have right bias?

October 26, 2018 at 12:00 pm

Hi Tetyana,

You’re very welcome!

The term “right bias” is not a standard term. Do you by chance mean right skewed distributions? In other words, if you plot the distribution for each group on a histogram they have longer right tails? These are not the symmetrical bell-shape curves of the normal distribution.

If that’s the case, yes you can as long as you exceed a specific sample size within each group. I include a table that contains these sample size requirements in my post about nonparametric vs parametric analyses .

Bias in statistics refers to cases where an estimate of a value is systematically higher or lower than the true value. If this is the case, you might be able to use t-tests, but you’d need to be sure to understand the nature of the bias so you would understand what the results are really indicating.

I hope this helps!

April 2, 2018 at 7:28 am

Simple and upto the point 👍 Thank you so much.

April 2, 2018 at 11:11 am

Hi Kalpana, thanks! And I’m glad it was helpful!

March 26, 2018 at 8:41 am

Am I correct if I say: Alpha – Probability of wrongly rejection of null hypothesis P-value – Probability of wrongly acceptance of null hypothesis

March 28, 2018 at 3:14 pm

You’re correct about alpha. Alpha is the probability of rejecting the null hypothesis when the null is true.

Unfortunately, your definition of the p-value is a bit off. The p-value has a fairly convoluted definition. It is the probability of obtaining the effect observed in a sample, or more extreme, if the null hypothesis is true. The p-value does NOT indicate the probability that either the null or alternative is true or false. Although, those are very common misinterpretations. To learn more, read my post about how to interpret p-values correctly .

March 2, 2018 at 6:10 pm

I recently started reading your blog and it is very helpful to understand each concept of statistical tests in easy way with some good examples. Also, I recommend to other people go through all these blogs which you posted. Specially for those people who have not statistical background and they are facing to many problems while studying statistical analysis.

Thank you for your such good blogs.

March 3, 2018 at 10:12 pm

Hi Amit, I’m so glad that my blog posts have been helpful for you! It means a lot to me that you took the time to write such a nice comment! Also, thanks for recommending by blog to others! I try really hard to write posts about statistics that are easy to understand.

January 17, 2018 at 7:03 am

I recently started reading your blog and I find it very interesting. I am learning statistics by my own, and I generally do many google search to understand the concepts. So this blog is quite helpful for me, as it have most of the content which I am looking for.

January 17, 2018 at 3:56 pm

Hi Shashank, thank you! And, I’m very glad to hear that my blog is helpful!

January 2, 2018 at 2:28 pm

thank u very much sir.

January 2, 2018 at 2:36 pm

You’re very welcome, Hiral!

November 21, 2017 at 12:43 pm

Thank u so much sir….your posts always helps me to be a #statistician

November 21, 2017 at 2:40 pm

Hi Sachin, you’re very welcome! I’m happy that you find my posts to be helpful!

November 19, 2017 at 8:22 pm

great post as usual, but it would be nice to see an example.

November 19, 2017 at 8:27 pm

Thank you! At the end of this post, I have links to four other posts that show examples of hypothesis tests in action. You’ll find what you’re looking for in those posts!

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Hypothesis Testing – A Deep Dive into Hypothesis Testing, The Backbone of Statistical Inference

• September 21, 2023

Explore the intricacies of hypothesis testing, a cornerstone of statistical analysis. Dive into methods, interpretations, and applications for making data-driven decisions.

In this Blog post we will learn:

• What is Hypothesis Testing?
• Steps in Hypothesis Testing 2.1. Set up Hypotheses: Null and Alternative 2.2. Choose a Significance Level (α) 2.3. Calculate a test statistic and P-Value 2.4. Make a Decision
• Example : Testing a new drug.
• Example in python

1. What is Hypothesis Testing?

In simple terms, hypothesis testing is a method used to make decisions or inferences about population parameters based on sample data. Imagine being handed a dice and asked if it’s biased. By rolling it a few times and analyzing the outcomes, you’d be engaging in the essence of hypothesis testing.

Think of hypothesis testing as the scientific method of the statistics world. Suppose you hear claims like “This new drug works wonders!” or “Our new website design boosts sales.” How do you know if these statements hold water? Enter hypothesis testing.

2. Steps in Hypothesis Testing

• Set up Hypotheses : Begin with a null hypothesis (H0) and an alternative hypothesis (Ha).
• Choose a Significance Level (α) : Typically 0.05, this is the probability of rejecting the null hypothesis when it’s actually true. Think of it as the chance of accusing an innocent person.
• Calculate Test statistic and P-Value : Gather evidence (data) and calculate a test statistic.
• p-value : This is the probability of observing the data, given that the null hypothesis is true. A small p-value (typically ≤ 0.05) suggests the data is inconsistent with the null hypothesis.
• Decision Rule : If the p-value is less than or equal to α, you reject the null hypothesis in favor of the alternative.

2.1. Set up Hypotheses: Null and Alternative

Before diving into testing, we must formulate hypotheses. The null hypothesis (H0) represents the default assumption, while the alternative hypothesis (H1) challenges it.

For instance, in drug testing, H0 : “The new drug is no better than the existing one,” H1 : “The new drug is superior .”

2.2. Choose a Significance Level (α)

When You collect and analyze data to test H0 and H1 hypotheses. Based on your analysis, you decide whether to reject the null hypothesis in favor of the alternative, or fail to reject / Accept the null hypothesis.

The significance level, often denoted by $α$, represents the probability of rejecting the null hypothesis when it is actually true.

In other words, it’s the risk you’re willing to take of making a Type I error (false positive).

Type I Error (False Positive) :

• Symbolized by the Greek letter alpha (α).
• Occurs when you incorrectly reject a true null hypothesis . In other words, you conclude that there is an effect or difference when, in reality, there isn’t.
• The probability of making a Type I error is denoted by the significance level of a test. Commonly, tests are conducted at the 0.05 significance level , which means there’s a 5% chance of making a Type I error .
• Commonly used significance levels are 0.01, 0.05, and 0.10, but the choice depends on the context of the study and the level of risk one is willing to accept.

Example : If a drug is not effective (truth), but a clinical trial incorrectly concludes that it is effective (based on the sample data), then a Type I error has occurred.

Type II Error (False Negative) :

• Symbolized by the Greek letter beta (β).
• Occurs when you accept a false null hypothesis . This means you conclude there is no effect or difference when, in reality, there is.
• The probability of making a Type II error is denoted by β. The power of a test (1 – β) represents the probability of correctly rejecting a false null hypothesis.

Example : If a drug is effective (truth), but a clinical trial incorrectly concludes that it is not effective (based on the sample data), then a Type II error has occurred.

Balancing the Errors :

In practice, there’s a trade-off between Type I and Type II errors. Reducing the risk of one typically increases the risk of the other. For example, if you want to decrease the probability of a Type I error (by setting a lower significance level), you might increase the probability of a Type II error unless you compensate by collecting more data or making other adjustments.

It’s essential to understand the consequences of both types of errors in any given context. In some situations, a Type I error might be more severe, while in others, a Type II error might be of greater concern. This understanding guides researchers in designing their experiments and choosing appropriate significance levels.

2.3. Calculate a test statistic and P-Value

Test statistic : A test statistic is a single number that helps us understand how far our sample data is from what we’d expect under a null hypothesis (a basic assumption we’re trying to test against). Generally, the larger the test statistic, the more evidence we have against our null hypothesis. It helps us decide whether the differences we observe in our data are due to random chance or if there’s an actual effect.

P-value : The P-value tells us how likely we would get our observed results (or something more extreme) if the null hypothesis were true. It’s a value between 0 and 1. – A smaller P-value (typically below 0.05) means that the observation is rare under the null hypothesis, so we might reject the null hypothesis. – A larger P-value suggests that what we observed could easily happen by random chance, so we might not reject the null hypothesis.

2.4. Make a Decision

Relationship between $α$ and P-Value

When conducting a hypothesis test:

• We first choose a significance level ($α$), which sets a threshold for making decisions.

We then calculate the p-value from our sample data and the test statistic.

Finally, we compare the p-value to our chosen $α$:

• If $p−value≤α$: We reject the null hypothesis in favor of the alternative hypothesis. The result is said to be statistically significant.
• If $p−value>α$: We fail to reject the null hypothesis. There isn’t enough statistical evidence to support the alternative hypothesis.

3. Example : Testing a new drug.

Imagine we are investigating whether a new drug is effective at treating headaches faster than drug B.

Setting Up the Experiment : You gather 100 people who suffer from headaches. Half of them (50 people) are given the new drug (let’s call this the ‘Drug Group’), and the other half are given a sugar pill, which doesn’t contain any medication.

• Set up Hypotheses : Before starting, you make a prediction:
• Null Hypothesis (H0): The new drug has no effect. Any difference in healing time between the two groups is just due to random chance.
• Alternative Hypothesis (H1): The new drug does have an effect. The difference in healing time between the two groups is significant and not just by chance.
• Choose a Significance Level (α) : Typically 0.05, this is the probability of rejecting the null hypothesis when it’s actually true

Calculate Test statistic and P-Value : After the experiment, you analyze the data. The “test statistic” is a number that helps you understand the difference between the two groups in terms of standard units.

For instance, let’s say:

• The average healing time in the Drug Group is 2 hours.
• The average healing time in the Placebo Group is 3 hours.

The test statistic helps you understand how significant this 1-hour difference is. If the groups are large and the spread of healing times in each group is small, then this difference might be significant. But if there’s a huge variation in healing times, the 1-hour difference might not be so special.

Imagine the P-value as answering this question: “If the new drug had NO real effect, what’s the probability that I’d see a difference as extreme (or more extreme) as the one I found, just by random chance?”

For instance:

• P-value of 0.01 means there’s a 1% chance that the observed difference (or a more extreme difference) would occur if the drug had no effect. That’s pretty rare, so we might consider the drug effective.
• P-value of 0.5 means there’s a 50% chance you’d see this difference just by chance. That’s pretty high, so we might not be convinced the drug is doing much.
• If the P-value is less than ($α$) 0.05: the results are “statistically significant,” and they might reject the null hypothesis , believing the new drug has an effect.
• If the P-value is greater than ($α$) 0.05: the results are not statistically significant, and they don’t reject the null hypothesis , remaining unsure if the drug has a genuine effect.

4. Example in python

For simplicity, let’s say we’re using a t-test (common for comparing means). Let’s dive into Python:

Making a Decision : “The results are statistically significant! p-value < 0.05 , The drug seems to have an effect!” If not, we’d say, “Looks like the drug isn’t as miraculous as we thought.”

5. Conclusion

Hypothesis testing is an indispensable tool in data science, allowing us to make data-driven decisions with confidence. By understanding its principles, conducting tests properly, and considering real-world applications, you can harness the power of hypothesis testing to unlock valuable insights from your data.

More Articles

F statistic formula – explained, correlation – connecting the dots, the role of correlation in data analysis, sampling and sampling distributions – a comprehensive guide on sampling and sampling distributions, law of large numbers – a deep dive into the world of statistics, central limit theorem – a deep dive into central limit theorem and its significance in statistics, similar articles, complete introduction to linear regression in r, how to implement common statistical significance tests and find the p value, logistic regression – a complete tutorial with examples in r.

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S.3 hypothesis testing.

In reviewing hypothesis tests, we start first with the general idea. Then, we keep returning to the basic procedures of hypothesis testing, each time adding a little more detail.

The general idea of hypothesis testing involves:

• Making an initial assumption.
• Collecting evidence (data).
• Based on the available evidence (data), deciding whether to reject or not reject the initial assumption.

Every hypothesis test — regardless of the population parameter involved — requires the above three steps.

Example S.3.1

Is normal body temperature really 98.6 degrees f section  .

Consider the population of many, many adults. A researcher hypothesized that the average adult body temperature is lower than the often-advertised 98.6 degrees F. That is, the researcher wants an answer to the question: "Is the average adult body temperature 98.6 degrees? Or is it lower?" To answer his research question, the researcher starts by assuming that the average adult body temperature was 98.6 degrees F.

Then, the researcher went out and tried to find evidence that refutes his initial assumption. In doing so, he selects a random sample of 130 adults. The average body temperature of the 130 sampled adults is 98.25 degrees.

Then, the researcher uses the data he collected to make a decision about his initial assumption. It is either likely or unlikely that the researcher would collect the evidence he did given his initial assumption that the average adult body temperature is 98.6 degrees:

• If it is likely , then the researcher does not reject his initial assumption that the average adult body temperature is 98.6 degrees. There is not enough evidence to do otherwise.
• either the researcher's initial assumption is correct and he experienced a very unusual event;
• or the researcher's initial assumption is incorrect.

In statistics, we generally don't make claims that require us to believe that a very unusual event happened. That is, in the practice of statistics, if the evidence (data) we collected is unlikely in light of the initial assumption, then we reject our initial assumption.

Example S.3.2

Criminal trial analogy section  .

One place where you can consistently see the general idea of hypothesis testing in action is in criminal trials held in the United States. Our criminal justice system assumes "the defendant is innocent until proven guilty." That is, our initial assumption is that the defendant is innocent.

In the practice of statistics, we make our initial assumption when we state our two competing hypotheses -- the null hypothesis ( H 0 ) and the alternative hypothesis ( H A ). Here, our hypotheses are:

• H 0 : Defendant is not guilty (innocent)
• H A : Defendant is guilty

In statistics, we always assume the null hypothesis is true . That is, the null hypothesis is always our initial assumption.

The prosecution team then collects evidence — such as finger prints, blood spots, hair samples, carpet fibers, shoe prints, ransom notes, and handwriting samples — with the hopes of finding "sufficient evidence" to make the assumption of innocence refutable.

In statistics, the data are the evidence.

The jury then makes a decision based on the available evidence:

• If the jury finds sufficient evidence — beyond a reasonable doubt — to make the assumption of innocence refutable, the jury rejects the null hypothesis and deems the defendant guilty. We behave as if the defendant is guilty.
• If there is insufficient evidence, then the jury does not reject the null hypothesis . We behave as if the defendant is innocent.

In statistics, we always make one of two decisions. We either "reject the null hypothesis" or we "fail to reject the null hypothesis."

Errors in Hypothesis Testing Section  

Did you notice the use of the phrase "behave as if" in the previous discussion? We "behave as if" the defendant is guilty; we do not "prove" that the defendant is guilty. And, we "behave as if" the defendant is innocent; we do not "prove" that the defendant is innocent.

This is a very important distinction! We make our decision based on evidence not on 100% guaranteed proof. Again:

• If we reject the null hypothesis, we do not prove that the alternative hypothesis is true.
• If we do not reject the null hypothesis, we do not prove that the null hypothesis is true.

We merely state that there is enough evidence to behave one way or the other. This is always true in statistics! Because of this, whatever the decision, there is always a chance that we made an error .

Let's review the two types of errors that can be made in criminal trials:

Table S.3.2 shows how this corresponds to the two types of errors in hypothesis testing.

Note that, in statistics, we call the two types of errors by two different  names -- one is called a "Type I error," and the other is called  a "Type II error." Here are the formal definitions of the two types of errors:

There is always a chance of making one of these errors. But, a good scientific study will minimize the chance of doing so!

Making the Decision Section  

Recall that it is either likely or unlikely that we would observe the evidence we did given our initial assumption. If it is likely , we do not reject the null hypothesis. If it is unlikely , then we reject the null hypothesis in favor of the alternative hypothesis. Effectively, then, making the decision reduces to determining "likely" or "unlikely."

In statistics, there are two ways to determine whether the evidence is likely or unlikely given the initial assumption:

• We could take the " critical value approach " (favored in many of the older textbooks).
• Or, we could take the " P -value approach " (what is used most often in research, journal articles, and statistical software).

In the next two sections, we review the procedures behind each of these two approaches. To make our review concrete, let's imagine that μ is the average grade point average of all American students who major in mathematics. We first review the critical value approach for conducting each of the following three hypothesis tests about the population mean $\mu$:

In Practice

• We would want to conduct the first hypothesis test if we were interested in concluding that the average grade point average of the group is more than 3.
• We would want to conduct the second hypothesis test if we were interested in concluding that the average grade point average of the group is less than 3.
• And, we would want to conduct the third hypothesis test if we were only interested in concluding that the average grade point average of the group differs from 3 (without caring whether it is more or less than 3).

Upon completing the review of the critical value approach, we review the P -value approach for conducting each of the above three hypothesis tests about the population mean \(\mu\). The procedures that we review here for both approaches easily extend to hypothesis tests about any other population parameter.

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• Knowledge Base

Methodology

• How to Write a Strong Hypothesis | Steps & Examples

How to Write a Strong Hypothesis | Steps & Examples

Published on May 6, 2022 by Shona McCombes . Revised on November 20, 2023.

A hypothesis is a statement that can be tested by scientific research. If you want to test a relationship between two or more variables, you need to write hypotheses before you start your experiment or data collection .

Example: Hypothesis

Daily apple consumption leads to fewer doctor’s visits.

Table of contents

What is a hypothesis, developing a hypothesis (with example), hypothesis examples, other interesting articles, frequently asked questions about writing hypotheses.

A hypothesis states your predictions about what your research will find. It is a tentative answer to your research question that has not yet been tested. For some research projects, you might have to write several hypotheses that address different aspects of your research question.

A hypothesis is not just a guess – it should be based on existing theories and knowledge. It also has to be testable, which means you can support or refute it through scientific research methods (such as experiments, observations and statistical analysis of data).

Variables in hypotheses

Hypotheses propose a relationship between two or more types of variables .

• An independent variable is something the researcher changes or controls.
• A dependent variable is something the researcher observes and measures.

If there are any control variables , extraneous variables , or confounding variables , be sure to jot those down as you go to minimize the chances that research bias  will affect your results.

In this example, the independent variable is exposure to the sun – the assumed cause . The dependent variable is the level of happiness – the assumed effect .

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Step 1. Ask a question

Writing a hypothesis begins with a research question that you want to answer. The question should be focused, specific, and researchable within the constraints of your project.

Step 2. Do some preliminary research

Your initial answer to the question should be based on what is already known about the topic. Look for theories and previous studies to help you form educated assumptions about what your research will find.

At this stage, you might construct a conceptual framework to ensure that you’re embarking on a relevant topic . This can also help you identify which variables you will study and what you think the relationships are between them. Sometimes, you’ll have to operationalize more complex constructs.

Step 3. Formulate your hypothesis

Now you should have some idea of what you expect to find. Write your initial answer to the question in a clear, concise sentence.

4. Refine your hypothesis

You need to make sure your hypothesis is specific and testable. There are various ways of phrasing a hypothesis, but all the terms you use should have clear definitions, and the hypothesis should contain:

• The relevant variables
• The specific group being studied
• The predicted outcome of the experiment or analysis

5. Phrase your hypothesis in three ways

To identify the variables, you can write a simple prediction in  if…then form. The first part of the sentence states the independent variable and the second part states the dependent variable.

In academic research, hypotheses are more commonly phrased in terms of correlations or effects, where you directly state the predicted relationship between variables.

If you are comparing two groups, the hypothesis can state what difference you expect to find between them.

6. Write a null hypothesis

If your research involves statistical hypothesis testing , you will also have to write a null hypothesis . The null hypothesis is the default position that there is no association between the variables. The null hypothesis is written as H 0 , while the alternative hypothesis is H 1 or H a .

• H 0 : The number of lectures attended by first-year students has no effect on their final exam scores.
• H 1 : The number of lectures attended by first-year students has a positive effect on their final exam scores.

If you want to know more about the research process , methodology , research bias , or statistics , make sure to check out some of our other articles with explanations and examples.

• Sampling methods
• Simple random sampling
• Stratified sampling
• Cluster sampling
• Likert scales
• Reproducibility

 Statistics

• Null hypothesis
• Statistical power
• Probability distribution
• Effect size
• Poisson distribution

Research bias

• Optimism bias
• Cognitive bias
• Implicit bias
• Hawthorne effect
• Anchoring bias
• Explicit bias

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A hypothesis is not just a guess — it should be based on existing theories and knowledge. It also has to be testable, which means you can support or refute it through scientific research methods (such as experiments, observations and statistical analysis of data).

Null and alternative hypotheses are used in statistical hypothesis testing . The null hypothesis of a test always predicts no effect or no relationship between variables, while the alternative hypothesis states your research prediction of an effect or relationship.

Hypothesis testing is a formal procedure for investigating our ideas about the world using statistics. It is used by scientists to test specific predictions, called hypotheses , by calculating how likely it is that a pattern or relationship between variables could have arisen by chance.

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McCombes, S. (2023, November 20). How to Write a Strong Hypothesis | Steps & Examples. Scribbr. Retrieved September 23, 2024, from https://www.scribbr.com/methodology/hypothesis/

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Hypothesis Testing

Hypothesis testing is a tool for making statistical inferences about the population data. It is an analysis tool that tests assumptions and determines how likely something is within a given standard of accuracy. Hypothesis testing provides a way to verify whether the results of an experiment are valid.

A null hypothesis and an alternative hypothesis are set up before performing the hypothesis testing. This helps to arrive at a conclusion regarding the sample obtained from the population. In this article, we will learn more about hypothesis testing, its types, steps to perform the testing, and associated examples.

What is Hypothesis Testing in Statistics?

Hypothesis testing uses sample data from the population to draw useful conclusions regarding the population probability distribution . It tests an assumption made about the data using different types of hypothesis testing methodologies. The hypothesis testing results in either rejecting or not rejecting the null hypothesis.

Hypothesis Testing Definition

Hypothesis testing can be defined as a statistical tool that is used to identify if the results of an experiment are meaningful or not. It involves setting up a null hypothesis and an alternative hypothesis. These two hypotheses will always be mutually exclusive. This means that if the null hypothesis is true then the alternative hypothesis is false and vice versa. An example of hypothesis testing is setting up a test to check if a new medicine works on a disease in a more efficient manner.

Null Hypothesis

The null hypothesis is a concise mathematical statement that is used to indicate that there is no difference between two possibilities. In other words, there is no difference between certain characteristics of data. This hypothesis assumes that the outcomes of an experiment are based on chance alone. It is denoted as \(H_{0}\). Hypothesis testing is used to conclude if the null hypothesis can be rejected or not. Suppose an experiment is conducted to check if girls are shorter than boys at the age of 5. The null hypothesis will say that they are the same height.

Alternative Hypothesis

The alternative hypothesis is an alternative to the null hypothesis. It is used to show that the observations of an experiment are due to some real effect. It indicates that there is a statistical significance between two possible outcomes and can be denoted as \(H_{1}\) or \(H_{a}\). For the above-mentioned example, the alternative hypothesis would be that girls are shorter than boys at the age of 5.

Hypothesis Testing P Value

In hypothesis testing, the p value is used to indicate whether the results obtained after conducting a test are statistically significant or not. It also indicates the probability of making an error in rejecting or not rejecting the null hypothesis.This value is always a number between 0 and 1. The p value is compared to an alpha level, \(\alpha\) or significance level. The alpha level can be defined as the acceptable risk of incorrectly rejecting the null hypothesis. The alpha level is usually chosen between 1% to 5%.

Hypothesis Testing Critical region

All sets of values that lead to rejecting the null hypothesis lie in the critical region. Furthermore, the value that separates the critical region from the non-critical region is known as the critical value.

Hypothesis Testing Formula

Depending upon the type of data available and the size, different types of hypothesis testing are used to determine whether the null hypothesis can be rejected or not. The hypothesis testing formula for some important test statistics are given below:

• z = \(\frac{\overline{x}-\mu}{\frac{\sigma}{\sqrt{n}}}\). \(\overline{x}\) is the sample mean, \(\mu\) is the population mean, \(\sigma\) is the population standard deviation and n is the size of the sample.
• t = \(\frac{\overline{x}-\mu}{\frac{s}{\sqrt{n}}}\). s is the sample standard deviation.
• \(\chi ^{2} = \sum \frac{(O_{i}-E_{i})^{2}}{E_{i}}\). \(O_{i}\) is the observed value and \(E_{i}\) is the expected value.

We will learn more about these test statistics in the upcoming section.

Types of Hypothesis Testing

Selecting the correct test for performing hypothesis testing can be confusing. These tests are used to determine a test statistic on the basis of which the null hypothesis can either be rejected or not rejected. Some of the important tests used for hypothesis testing are given below.

Hypothesis Testing Z Test

A z test is a way of hypothesis testing that is used for a large sample size (n ≥ 30). It is used to determine whether there is a difference between the population mean and the sample mean when the population standard deviation is known. It can also be used to compare the mean of two samples. It is used to compute the z test statistic. The formulas are given as follows:

• One sample: z = \(\frac{\overline{x}-\mu}{\frac{\sigma}{\sqrt{n}}}\).
• Two samples: z = \(\frac{(\overline{x_{1}}-\overline{x_{2}})-(\mu_{1}-\mu_{2})}{\sqrt{\frac{\sigma_{1}^{2}}{n_{1}}+\frac{\sigma_{2}^{2}}{n_{2}}}}\).

Hypothesis Testing t Test

The t test is another method of hypothesis testing that is used for a small sample size (n < 30). It is also used to compare the sample mean and population mean. However, the population standard deviation is not known. Instead, the sample standard deviation is known. The mean of two samples can also be compared using the t test.

• One sample: t = \(\frac{\overline{x}-\mu}{\frac{s}{\sqrt{n}}}\).
• Two samples: t = \(\frac{(\overline{x_{1}}-\overline{x_{2}})-(\mu_{1}-\mu_{2})}{\sqrt{\frac{s_{1}^{2}}{n_{1}}+\frac{s_{2}^{2}}{n_{2}}}}\).

Hypothesis Testing Chi Square

The Chi square test is a hypothesis testing method that is used to check whether the variables in a population are independent or not. It is used when the test statistic is chi-squared distributed.

One Tailed Hypothesis Testing

One tailed hypothesis testing is done when the rejection region is only in one direction. It can also be known as directional hypothesis testing because the effects can be tested in one direction only. This type of testing is further classified into the right tailed test and left tailed test.

Right Tailed Hypothesis Testing

The right tail test is also known as the upper tail test. This test is used to check whether the population parameter is greater than some value. The null and alternative hypotheses for this test are given as follows:

\(H_{0}\): The population parameter is ≤ some value

\(H_{1}\): The population parameter is > some value.

If the test statistic has a greater value than the critical value then the null hypothesis is rejected

Left Tailed Hypothesis Testing

The left tail test is also known as the lower tail test. It is used to check whether the population parameter is less than some value. The hypotheses for this hypothesis testing can be written as follows:

\(H_{0}\): The population parameter is ≥ some value

\(H_{1}\): The population parameter is < some value.

The null hypothesis is rejected if the test statistic has a value lesser than the critical value.

Two Tailed Hypothesis Testing

In this hypothesis testing method, the critical region lies on both sides of the sampling distribution. It is also known as a non - directional hypothesis testing method. The two-tailed test is used when it needs to be determined if the population parameter is assumed to be different than some value. The hypotheses can be set up as follows:

\(H_{0}\): the population parameter = some value

\(H_{1}\): the population parameter ≠ some value

The null hypothesis is rejected if the test statistic has a value that is not equal to the critical value.

Hypothesis Testing Steps

Hypothesis testing can be easily performed in five simple steps. The most important step is to correctly set up the hypotheses and identify the right method for hypothesis testing. The basic steps to perform hypothesis testing are as follows:

• Step 1: Set up the null hypothesis by correctly identifying whether it is the left-tailed, right-tailed, or two-tailed hypothesis testing.
• Step 2: Set up the alternative hypothesis.
• Step 3: Choose the correct significance level, \(\alpha\), and find the critical value.
• Step 4: Calculate the correct test statistic (z, t or \(\chi\)) and p-value.
• Step 5: Compare the test statistic with the critical value or compare the p-value with \(\alpha\) to arrive at a conclusion. In other words, decide if the null hypothesis is to be rejected or not.

Hypothesis Testing Example

The best way to solve a problem on hypothesis testing is by applying the 5 steps mentioned in the previous section. Suppose a researcher claims that the mean average weight of men is greater than 100kgs with a standard deviation of 15kgs. 30 men are chosen with an average weight of 112.5 Kgs. Using hypothesis testing, check if there is enough evidence to support the researcher's claim. The confidence interval is given as 95%.

Step 1: This is an example of a right-tailed test. Set up the null hypothesis as \(H_{0}\): \(\mu\) = 100.

Step 2: The alternative hypothesis is given by \(H_{1}\): \(\mu\) > 100.

Step 3: As this is a one-tailed test, \(\alpha\) = 100% - 95% = 5%. This can be used to determine the critical value.

1 - \(\alpha\) = 1 - 0.05 = 0.95

0.95 gives the required area under the curve. Now using a normal distribution table, the area 0.95 is at z = 1.645. A similar process can be followed for a t-test. The only additional requirement is to calculate the degrees of freedom given by n - 1.

Step 4: Calculate the z test statistic. This is because the sample size is 30. Furthermore, the sample and population means are known along with the standard deviation.

z = \(\frac{\overline{x}-\mu}{\frac{\sigma}{\sqrt{n}}}\).

\(\mu\) = 100, \(\overline{x}\) = 112.5, n = 30, \(\sigma\) = 15

z = \(\frac{112.5-100}{\frac{15}{\sqrt{30}}}\) = 4.56

Step 5: Conclusion. As 4.56 > 1.645 thus, the null hypothesis can be rejected.

Hypothesis Testing and Confidence Intervals

Confidence intervals form an important part of hypothesis testing. This is because the alpha level can be determined from a given confidence interval. Suppose a confidence interval is given as 95%. Subtract the confidence interval from 100%. This gives 100 - 95 = 5% or 0.05. This is the alpha value of a one-tailed hypothesis testing. To obtain the alpha value for a two-tailed hypothesis testing, divide this value by 2. This gives 0.05 / 2 = 0.025.

Related Articles:

• Probability and Statistics
• Data Handling

Important Notes on Hypothesis Testing

• Hypothesis testing is a technique that is used to verify whether the results of an experiment are statistically significant.
• It involves the setting up of a null hypothesis and an alternate hypothesis.
• There are three types of tests that can be conducted under hypothesis testing - z test, t test, and chi square test.
• Hypothesis testing can be classified as right tail, left tail, and two tail tests.

Examples on Hypothesis Testing

• Example 1: The average weight of a dumbbell in a gym is 90lbs. However, a physical trainer believes that the average weight might be higher. A random sample of 5 dumbbells with an average weight of 110lbs and a standard deviation of 18lbs. Using hypothesis testing check if the physical trainer's claim can be supported for a 95% confidence level. Solution: As the sample size is lesser than 30, the t-test is used. \(H_{0}\): \(\mu\) = 90, \(H_{1}\): \(\mu\) > 90 \(\overline{x}\) = 110, \(\mu\) = 90, n = 5, s = 18. \(\alpha\) = 0.05 Using the t-distribution table, the critical value is 2.132 t = \(\frac{\overline{x}-\mu}{\frac{s}{\sqrt{n}}}\) t = 2.484 As 2.484 > 2.132, the null hypothesis is rejected. Answer: The average weight of the dumbbells may be greater than 90lbs
• Example 2: The average score on a test is 80 with a standard deviation of 10. With a new teaching curriculum introduced it is believed that this score will change. On random testing, the score of 38 students, the mean was found to be 88. With a 0.05 significance level, is there any evidence to support this claim? Solution: This is an example of two-tail hypothesis testing. The z test will be used. \(H_{0}\): \(\mu\) = 80, \(H_{1}\): \(\mu\) ≠ 80 \(\overline{x}\) = 88, \(\mu\) = 80, n = 36, \(\sigma\) = 10. \(\alpha\) = 0.05 / 2 = 0.025 The critical value using the normal distribution table is 1.96 z = \(\frac{\overline{x}-\mu}{\frac{\sigma}{\sqrt{n}}}\) z = \(\frac{88-80}{\frac{10}{\sqrt{36}}}\) = 4.8 As 4.8 > 1.96, the null hypothesis is rejected. Answer: There is a difference in the scores after the new curriculum was introduced.
• Example 3: The average score of a class is 90. However, a teacher believes that the average score might be lower. The scores of 6 students were randomly measured. The mean was 82 with a standard deviation of 18. With a 0.05 significance level use hypothesis testing to check if this claim is true. Solution: The t test will be used. \(H_{0}\): \(\mu\) = 90, \(H_{1}\): \(\mu\) < 90 \(\overline{x}\) = 110, \(\mu\) = 90, n = 6, s = 18 The critical value from the t table is -2.015 t = \(\frac{\overline{x}-\mu}{\frac{s}{\sqrt{n}}}\) t = \(\frac{82-90}{\frac{18}{\sqrt{6}}}\) t = -1.088 As -1.088 > -2.015, we fail to reject the null hypothesis. Answer: There is not enough evidence to support the claim.

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FAQs on Hypothesis Testing

What is hypothesis testing.

Hypothesis testing in statistics is a tool that is used to make inferences about the population data. It is also used to check if the results of an experiment are valid.

What is the z Test in Hypothesis Testing?

The z test in hypothesis testing is used to find the z test statistic for normally distributed data . The z test is used when the standard deviation of the population is known and the sample size is greater than or equal to 30.

What is the t Test in Hypothesis Testing?

The t test in hypothesis testing is used when the data follows a student t distribution . It is used when the sample size is less than 30 and standard deviation of the population is not known.

What is the formula for z test in Hypothesis Testing?

The formula for a one sample z test in hypothesis testing is z = \(\frac{\overline{x}-\mu}{\frac{\sigma}{\sqrt{n}}}\) and for two samples is z = \(\frac{(\overline{x_{1}}-\overline{x_{2}})-(\mu_{1}-\mu_{2})}{\sqrt{\frac{\sigma_{1}^{2}}{n_{1}}+\frac{\sigma_{2}^{2}}{n_{2}}}}\).

What is the p Value in Hypothesis Testing?

The p value helps to determine if the test results are statistically significant or not. In hypothesis testing, the null hypothesis can either be rejected or not rejected based on the comparison between the p value and the alpha level.

What is One Tail Hypothesis Testing?

When the rejection region is only on one side of the distribution curve then it is known as one tail hypothesis testing. The right tail test and the left tail test are two types of directional hypothesis testing.

What is the Alpha Level in Two Tail Hypothesis Testing?

To get the alpha level in a two tail hypothesis testing divide \(\alpha\) by 2. This is done as there are two rejection regions in the curve.

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Lesson 10 of 24 By Avijeet Biswal

Table of Contents

In today’s data-driven world, decisions are based on data all the time. Hypothesis plays a crucial role in that process, whether it may be making business decisions, in the health sector, academia, or in quality improvement. Without hypothesis and hypothesis tests, you risk drawing the wrong conclusions and making bad decisions. In this tutorial, you will look at Hypothesis Testing in Statistics.

What Is Hypothesis Testing in Statistics?

Hypothesis Testing is a type of statistical analysis in which you put your assumptions about a population parameter to the test. It is used to estimate the relationship between 2 statistical variables.

Let's discuss few examples of statistical hypothesis from real-life - 

• A teacher assumes that 60% of his college's students come from lower-middle-class families.
• A doctor believes that 3D (Diet, Dose, and Discipline) is 90% effective for diabetic patients.

Now that you know about hypothesis testing, look at the two types of hypothesis testing in statistics.

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Importance of Hypothesis Testing in Data Analysis

Here is what makes hypothesis testing so important in data analysis and why it is key to making better decisions:

Avoiding Misleading Conclusions (Type I and Type II Errors)

One of the biggest benefits of hypothesis testing is that it helps you avoid jumping to the wrong conclusions. For instance, a Type I error could occur if a company launches a new product thinking it will be a hit, only to find out later that the data misled them. A Type II error might happen when a company overlooks a potentially successful product because their testing wasn’t thorough enough. By setting up the right significance level and carefully calculating the p-value, hypothesis testing minimizes the chances of these errors, leading to more accurate results.

Making Smarter Choices

Hypothesis testing is key to making smarter, evidence-based decisions. Let’s say a city planner wants to determine if building a new park will increase community engagement. By testing the hypothesis using data from similar projects, they can make an informed choice. Similarly, a teacher might use hypothesis testing to see if a new teaching method actually improves student performance. It’s about taking the guesswork out of decisions and relying on solid evidence instead.

Optimizing Business Tactics

In business, hypothesis testing is invaluable for testing new ideas and strategies before fully committing to them. For example, an e-commerce company might want to test whether offering free shipping increases sales. By using hypothesis testing, they can compare sales data from customers who received free shipping offers and those who didn’t. This allows them to base their business decisions on data, not hunches, reducing the risk of costly mistakes.

Hypothesis Testing Formula

Z = ( x̅ – μ0 ) / (σ /√n)

• Here, x̅ is the sample mean,
• μ0 is the population mean,
• σ is the standard deviation,
• n is the sample size.

How Hypothesis Testing Works?

An analyst performs hypothesis testing on a statistical sample to present evidence of the plausibility of the null hypothesis. Measurements and analyses are conducted on a random sample of the population to test a theory. Analysts use a random population sample to test two hypotheses: the null and alternative hypotheses.

The null hypothesis is typically an equality hypothesis between population parameters; for example, a null hypothesis may claim that the population means return equals zero. The alternate hypothesis is essentially the inverse of the null hypothesis (e.g., the population means the return is not equal to zero). As a result, they are mutually exclusive, and only one can be correct. One of the two possibilities, however, will always be correct.

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Null Hypothesis and Alternative Hypothesis

The Null Hypothesis is the assumption that the event will not occur. A null hypothesis has no bearing on the study's outcome unless it is rejected.

H0 is the symbol for it, and it is pronounced H-naught.

The Alternate Hypothesis is the logical opposite of the null hypothesis. The acceptance of the alternative hypothesis follows the rejection of the null hypothesis. H1 is the symbol for it.

Let's understand this with an example.

A sanitizer manufacturer claims that its product kills 95 percent of germs on average. 

To put this company's claim to the test, create a null and alternate hypothesis.

H0 (Null Hypothesis): Average = 95%.

Alternative Hypothesis (H1): The average is less than 95%.

Another straightforward example to understand this concept is determining whether or not a coin is fair and balanced. The null hypothesis states that the probability of a show of heads is equal to the likelihood of a show of tails. In contrast, the alternate theory states that the probability of a show of heads and tails would be very different.

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Hypothesis Testing Calculation With Examples

Let's consider a hypothesis test for the average height of women in the United States. Suppose our null hypothesis is that the average height is 5'4". We gather a sample of 100 women and determine their average height is 5'5". The standard deviation of population is 2.

To calculate the z-score, we would use the following formula:

z = ( x̅ – μ0 ) / (σ /√n)

z = (5'5" - 5'4") / (2" / √100)

z = 0.5 / (0.045)

We will reject the null hypothesis as the z-score of 11.11 is very large and conclude that there is evidence to suggest that the average height of women in the US is greater than 5'4".

Steps in Hypothesis Testing

Hypothesis testing is a statistical method to determine if there is enough evidence in a sample of data to infer that a certain condition is true for the entire population. Here’s a breakdown of the typical steps involved in hypothesis testing:

Formulate Hypotheses

• Null Hypothesis (H0): This hypothesis states that there is no effect or difference, and it is the hypothesis you attempt to reject with your test.
• Alternative Hypothesis (H1 or Ha): This hypothesis is what you might believe to be true or hope to prove true. It is usually considered the opposite of the null hypothesis.

Choose the Significance Level (α)

The significance level, often denoted by alpha (α), is the probability of rejecting the null hypothesis when it is true. Common choices for α are 0.05 (5%), 0.01 (1%), and 0.10 (10%).

Select the Appropriate Test

Choose a statistical test based on the type of data and the hypothesis. Common tests include t-tests, chi-square tests, ANOVA, and regression analysis. The selection depends on data type, distribution, sample size, and whether the hypothesis is one-tailed or two-tailed.

Collect Data

Gather the data that will be analyzed in the test. To infer conclusions accurately, this data should be representative of the population.

Calculate the Test Statistic

Based on the collected data and the chosen test, calculate a test statistic that reflects how much the observed data deviates from the null hypothesis.

Determine the p-value

The p-value is the probability of observing test results at least as extreme as the results observed, assuming the null hypothesis is correct. It helps determine the strength of the evidence against the null hypothesis.

Make a Decision

Compare the p-value to the chosen significance level:

• If the p-value ≤ α: Reject the null hypothesis, suggesting sufficient evidence in the data supports the alternative hypothesis.
• If the p-value > α: Do not reject the null hypothesis, suggesting insufficient evidence to support the alternative hypothesis.

Report the Results

Present the findings from the hypothesis test, including the test statistic, p-value, and the conclusion about the hypotheses.

Perform Post-hoc Analysis (if necessary)

Depending on the results and the study design, further analysis may be needed to explore the data more deeply or to address multiple comparisons if several hypotheses were tested simultaneously.

Types of Hypothesis Testing

To determine whether a discovery or relationship is statistically significant, hypothesis testing uses a z-test. It usually checks to see if two means are the same (the null hypothesis). Only when the population standard deviation is known and the sample size is 30 data points or more, can a z-test be applied.

A statistical test called a t-test is employed to compare the means of two groups. To determine whether two groups differ or if a procedure or treatment affects the population of interest, it is frequently used in hypothesis testing.

3. Chi-Square 

You utilize a Chi-square test for hypothesis testing concerning whether your data is as predicted. To determine if the expected and observed results are well-fitted, the Chi-square test analyzes the differences between categorical variables from a random sample. The test's fundamental premise is that the observed values in your data should be compared to the predicted values that would be present if the null hypothesis were true.

ANOVA , or Analysis of Variance, is a statistical method used to compare the means of three or more groups. It’s particularly useful when you want to see if there are significant differences between multiple groups. For instance, in business, a company might use ANOVA to analyze whether three different stores are performing differently in terms of sales. It’s also widely used in fields like medical research and social sciences, where comparing group differences can provide valuable insights.

Hypothesis Testing and Confidence Intervals

Both confidence intervals and hypothesis tests are inferential techniques that depend on approximating the sample distribution. Data from a sample is used to estimate a population parameter using confidence intervals. Data from a sample is used in hypothesis testing to examine a given hypothesis. We must have a postulated parameter to conduct hypothesis testing.

Bootstrap distributions and randomization distributions are created using comparable simulation techniques. The observed sample statistic is the focal point of a bootstrap distribution, whereas the null hypothesis value is the focal point of a randomization distribution.

A variety of feasible population parameter estimates are included in confidence ranges. In this lesson, we created just two-tailed confidence intervals. There is a direct connection between these two-tail confidence intervals and these two-tail hypothesis tests. The results of a two-tailed hypothesis test and two-tailed confidence intervals typically provide the same results. In other words, a hypothesis test at the 0.05 level will virtually always fail to reject the null hypothesis if the 95% confidence interval contains the predicted value. A hypothesis test at the 0.05 level will nearly certainly reject the null hypothesis if the 95% confidence interval does not include the hypothesized parameter.

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Simple and Composite Hypothesis Testing

Depending on the population distribution, you can classify the statistical hypothesis into two types.

Simple Hypothesis: A simple hypothesis specifies an exact value for the parameter.

Composite Hypothesis: A composite hypothesis specifies a range of values.

A company is claiming that their average sales for this quarter are 1000 units. This is an example of a simple hypothesis.

Suppose the company claims that the sales are in the range of 900 to 1000 units. Then this is a case of a composite hypothesis.

One-Tailed and Two-Tailed Hypothesis Testing

The One-Tailed test, also called a directional test, considers a critical region of data that would result in the null hypothesis being rejected if the test sample falls into it, inevitably meaning the acceptance of the alternate hypothesis.

In a one-tailed test, the critical distribution area is one-sided, meaning the test sample is either greater or lesser than a specific value.

In two tails, the test sample is checked to be greater or less than a range of values in a Two-Tailed test, implying that the critical distribution area is two-sided.

If the sample falls within this range, the alternate hypothesis will be accepted, and the null hypothesis will be rejected.

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Right Tailed Hypothesis Testing

If the larger than (>) sign appears in your hypothesis statement, you are using a right-tailed test, also known as an upper test. Or, to put it another way, the disparity is to the right. For instance, you can contrast the battery life before and after a change in production. Your hypothesis statements can be the following if you want to know if the battery life is longer than the original (let's say 90 hours):

• The null hypothesis is (H0 <= 90) or less change.
• A possibility is that battery life has risen (H1) > 90.

The crucial point in this situation is that the alternate hypothesis (H1), not the null hypothesis, decides whether you get a right-tailed test.

Left Tailed Hypothesis Testing

Alternative hypotheses that assert the true value of a parameter is lower than the null hypothesis are tested with a left-tailed test; they are indicated by the asterisk "<".

Suppose H0: mean = 50 and H1: mean not equal to 50

According to the H1, the mean can be greater than or less than 50. This is an example of a Two-tailed test.

In a similar manner, if H0: mean >=50, then H1: mean <50

Here the mean is less than 50. It is called a One-tailed test.

Type 1 and Type 2 Error

A hypothesis test can result in two types of errors.

Type 1 Error: A Type-I error occurs when sample results reject the null hypothesis despite being true.

Type 2 Error: A Type-II error occurs when the null hypothesis is not rejected when it is false, unlike a Type-I error.

Suppose a teacher evaluates the examination paper to decide whether a student passes or fails.

H0: Student has passed

H1: Student has failed

Type I error will be the teacher failing the student [rejects H0] although the student scored the passing marks [H0 was true]. 

Type II error will be the case where the teacher passes the student [do not reject H0] although the student did not score the passing marks [H1 is true].

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Practice Problems on Hypothesis Testing

Here are the practice problems on hypothesis testing that will help you understand how to apply these concepts in real-world scenarios:

A telecom service provider claims that customers spend an average of ₹400 per month, with a standard deviation of ₹25. However, a random sample of 50 customer bills shows a mean of ₹250 and a standard deviation of ₹15. Does this sample data support the service provider’s claim?

Solution: Let’s break this down:

• Null Hypothesis (H0): The average amount spent per month is ₹400.
• Alternate Hypothesis (H1): The average amount spent per month is not ₹400.
• Population Standard Deviation (σ): ₹25
• Sample Size (n): 50
• Sample Mean (x̄): ₹250

1. Calculate the z-value:

z=250-40025/50 −42.42

2. Compare with critical z-values: For a 5% significance level, critical z-values are -1.96 and +1.96. Since -42.42 is far outside this range, we reject the null hypothesis. The sample data suggests that the average amount spent is significantly different from ₹400.

Out of 850 customers, 400 made online grocery purchases. Can we conclude that more than 50% of customers are moving towards online grocery shopping?

Solution: Here’s how to approach it:

• Proportion of customers who shopped online (p): 400 / 850 = 0.47
• Null Hypothesis (H0): The proportion of online shoppers is 50% or more.
• Alternate Hypothesis (H1): The proportion of online shoppers is less than 50%.
• Sample Size (n): 850
• Significance Level (α): 5%

z=p-PP(1-P)/n

z=0.47-0.500.50.5/850  −1.74

2. Compare with the critical z-value: For a 5% significance level (one-tailed test), the critical z-value is -1.645. Since -1.74 is less than -1.645, we reject the null hypothesis. This means the data does not support the idea that most customers are moving towards online grocery shopping.

In a study of code quality, Team A has 250 errors in 1000 lines of code, and Team B has 300 errors in 800 lines of code. Can we say Team B performs worse than Team A?

Solution: Let’s analyze it:

• Proportion of errors for Team A (pA): 250 / 1000 = 0.25
• Proportion of errors for Team B (pB): 300 / 800 = 0.375
• Null Hypothesis (H0): Team B’s error rate is less than or equal to Team A’s.
• Alternate Hypothesis (H1): Team B’s error rate is greater than Team A’s.
• Sample Size for Team A (nA): 1000
• Sample Size for Team B (nB): 800

p=nApA+nBpBnA+nB

p=10000.25+8000.3751000+800 ≈ 0.305

z=​pA−pB​p(1-p)(1nA+1nB)

z=​0.25−0.375​0.305(1-0.305) (11000+1800) ≈ −5.72

2. Compare with the critical z-value: For a 5% significance level (one-tailed test), the critical z-value is +1.645. Since -5.72 is far less than +1.645, we reject the null hypothesis. The data indicates that Team B’s performance is significantly worse than Team A’s.

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Applications of Hypothesis Testing

Apart from the practical problems, let's look at the real-world applications of hypothesis testing across various fields:

Medicine and Healthcare

In medicine, hypothesis testing plays a pivotal role in assessing the success of new treatments. For example, researchers may want to find out if a new exercise regimen improves heart health. By comparing data from patients who followed the program to those who didn’t, they can determine if the exercise significantly improves health outcomes. Such rigorous testing allows medical professionals to rely on proven methods rather than assumptions.

Quality Control and Manufacturing

In manufacturing, ensuring product quality is vital, and hypothesis testing helps maintain those standards. Suppose a beverage company introduces a new bottling process and wants to verify if it reduces contamination. By analyzing samples from the new and old processes, hypothesis testing can reveal whether the new method reduces the risk of contamination. This allows manufacturers to implement improvements that enhance product safety and quality confidently.

Education and Learning

In education and learning, hypothesis testing is a tool to evaluate the impact of innovative teaching techniques. Imagine a situation where teachers introduce project-based learning to boost critical thinking skills. By comparing the performance of students who engaged in project-based learning with those in traditional settings, educators can test their hypothesis. The results can help educators make informed choices about adopting new teaching strategies.

Environmental Science

Hypothesis testing is essential in environmental science for evaluating the effectiveness of conservation measures. For example, scientists might explore whether a new water management strategy improves river health. By collecting and comparing data on water quality before and after the implementation of the strategy, they can determine whether the intervention leads to positive changes. Such findings are crucial for guiding environmental decisions that have long-term impacts.

Marketing and Advertising

In marketing, businesses use hypothesis testing to refine their approaches. For instance, a clothing brand might test if offering limited-time discounts increases customer loyalty. By running campaigns with and without the discount and analyzing the outcomes, they can assess if the strategy boosts customer retention. Data-driven insights from hypothesis testing enable companies to design marketing strategies that resonate with their audience and drive growth.

Limitations of Hypothesis Testing

Hypothesis testing has some limitations that researchers should be aware of:

• It cannot prove or establish the truth: Hypothesis testing provides evidence to support or reject a hypothesis, but it cannot confirm the absolute truth of the research question.
• Results are sample-specific: Hypothesis testing is based on analyzing a sample from a population, and the conclusions drawn are specific to that particular sample.
• Possible errors: During hypothesis testing, there is a chance of committing type I error (rejecting a true null hypothesis) or type II error (failing to reject a false null hypothesis).
• Assumptions and requirements: Different tests have specific assumptions and requirements that must be met to accurately interpret results.

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After reading this tutorial, you would have a much better understanding of hypothesis testing, one of the most important concepts in the field of Data Science . The majority of hypotheses are based on speculation about observed behavior, natural phenomena, or established theories.

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1. What is hypothesis testing in statistics with example?

Hypothesis testing is a statistical method used to determine if there is enough evidence in a sample data to draw conclusions about a population. It involves formulating two competing hypotheses, the null hypothesis (H0) and the alternative hypothesis (Ha), and then collecting data to assess the evidence. An example: testing if a new drug improves patient recovery (Ha) compared to the standard treatment (H0) based on collected patient data.

2. What is H0 and H1 in statistics?

In statistics, H0​ and H1​ represent the null and alternative hypotheses. The null hypothesis, H0​, is the default assumption that no effect or difference exists between groups or conditions. The alternative hypothesis, H1​, is the competing claim suggesting an effect or a difference. Statistical tests determine whether to reject the null hypothesis in favor of the alternative hypothesis based on the data.

3. What is a simple hypothesis with an example?

A simple hypothesis is a specific statement predicting a single relationship between two variables. It posits a direct and uncomplicated outcome. For example, a simple hypothesis might state, "Increased sunlight exposure increases the growth rate of sunflowers." Here, the hypothesis suggests a direct relationship between the amount of sunlight (independent variable) and the growth rate of sunflowers (dependent variable), with no additional variables considered.

4. What are the 3 major types of hypothesis?

The three major types of hypotheses are:

• Null Hypothesis (H0): Represents the default assumption, stating that there is no significant effect or relationship in the data.
• Alternative Hypothesis (Ha): Contradicts the null hypothesis and proposes a specific effect or relationship that researchers want to investigate.
• Nondirectional Hypothesis: An alternative hypothesis that doesn't specify the direction of the effect, leaving it open for both positive and negative possibilities.

5. What software tools can assist with hypothesis testing?

Several software tools offering distinct features can help with hypothesis testing. R and RStudio are popular for their advanced statistical capabilities. The Python ecosystem, including libraries like SciPy and Statsmodels, also supports hypothesis testing. SAS and SPSS are well-established tools for comprehensive statistical analysis. For basic testing, Excel offers simple built-in functions.

6. How do I interpret the results of a hypothesis test?

Interpreting hypothesis test results involves comparing the p-value to the significance level (alpha). If the p-value is less than or equal to alpha, you can reject the null hypothesis, indicating statistical significance. This suggests that the observed effect is unlikely to have occurred by chance, validating your analysis findings.

7. Why is sample size important in hypothesis testing?

Sample size is crucial in hypothesis testing as it affects the test’s power. A larger sample size increases the likelihood of detecting a true effect, reducing the risk of Type II errors. Conversely, a small sample may lack the statistical power needed to identify differences, potentially leading to inaccurate conclusions.

8. Can hypothesis testing be used for non-numerical data?

Yes, hypothesis testing can be applied to non-numerical data through non-parametric tests. These tests are ideal when data doesn't meet parametric assumptions or when dealing with categorical data. Non-parametric tests, like the Chi-square or Mann-Whitney U test, provide robust methods for analyzing non-numerical data and drawing meaningful conclusions.

9. How do I choose the proper hypothesis test?

Selecting the right hypothesis test depends on several factors: the objective of your analysis, the type of data (numerical or categorical), and the sample size. Consider whether you're comparing means, proportions, or associations, and whether your data follows a normal distribution. The correct choice ensures accurate results tailored to your research question.

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About the Author

Avijeet is a Senior Research Analyst at Simplilearn. Passionate about Data Analytics, Machine Learning, and Deep Learning, Avijeet is also interested in politics, cricket, and football.

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Hypothesis testing, p values, confidence intervals, and significance.

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• Definition/Introduction

Medical providers often rely on evidence-based medicine to guide decision-making in practice. Often a research hypothesis is tested with results provided, typically with p values, confidence intervals, or both. Additionally, statistical or research significance is estimated or determined by the investigators. Unfortunately, healthcare providers may have different comfort levels in interpreting these findings, which may affect the adequate application of the data.

• Issues of Concern

Without a foundational understanding of hypothesis testing, p values, confidence intervals, and the difference between statistical and clinical significance, it may affect healthcare providers' ability to make clinical decisions without relying purely on the research investigators deemed level of significance. Therefore, an overview of these concepts is provided to allow medical professionals to use their expertise to determine if results are reported sufficiently and if the study outcomes are clinically appropriate to be applied in healthcare practice.

Hypothesis Testing

Investigators conducting studies need research questions and hypotheses to guide analyses. Starting with broad research questions (RQs), investigators then identify a gap in current clinical practice or research. Any research problem or statement is grounded in a better understanding of relationships between two or more variables. For this article, we will use the following research question example:

Research Question: Is Drug 23 an effective treatment for Disease A?

Research questions do not directly imply specific guesses or predictions; we must formulate research hypotheses. A hypothesis is a predetermined declaration regarding the research question in which the investigator(s) makes a precise, educated guess about a study outcome. This is sometimes called the alternative hypothesis and ultimately allows the researcher to take a stance based on experience or insight from medical literature. An example of a hypothesis is below.

Research Hypothesis: Drug 23 will significantly reduce symptoms associated with Disease A compared to Drug 22.

The null hypothesis states that there is no statistical difference between groups based on the stated research hypothesis.

Researchers should be aware of journal recommendations when considering how to report p values, and manuscripts should remain internally consistent.

Regarding p values, as the number of individuals enrolled in a study (the sample size) increases, the likelihood of finding a statistically significant effect increases. With very large sample sizes, the p-value can be very low significant differences in the reduction of symptoms for Disease A between Drug 23 and Drug 22. The null hypothesis is deemed true until a study presents significant data to support rejecting the null hypothesis. Based on the results, the investigators will either reject the null hypothesis (if they found significant differences or associations) or fail to reject the null hypothesis (they could not provide proof that there were significant differences or associations).

To test a hypothesis, researchers obtain data on a representative sample to determine whether to reject or fail to reject a null hypothesis. In most research studies, it is not feasible to obtain data for an entire population. Using a sampling procedure allows for statistical inference, though this involves a certain possibility of error. [1]  When determining whether to reject or fail to reject the null hypothesis, mistakes can be made: Type I and Type II errors. Though it is impossible to ensure that these errors have not occurred, researchers should limit the possibilities of these faults. [2]

Significance

Significance is a term to describe the substantive importance of medical research. Statistical significance is the likelihood of results due to chance. [3]  Healthcare providers should always delineate statistical significance from clinical significance, a common error when reviewing biomedical research. [4]  When conceptualizing findings reported as either significant or not significant, healthcare providers should not simply accept researchers' results or conclusions without considering the clinical significance. Healthcare professionals should consider the clinical importance of findings and understand both p values and confidence intervals so they do not have to rely on the researchers to determine the level of significance. [5]  One criterion often used to determine statistical significance is the utilization of p values.

P values are used in research to determine whether the sample estimate is significantly different from a hypothesized value. The p-value is the probability that the observed effect within the study would have occurred by chance if, in reality, there was no true effect. Conventionally, data yielding a p<0.05 or p<0.01 is considered statistically significant. While some have debated that the 0.05 level should be lowered, it is still universally practiced. [6]  Hypothesis testing allows us to determine the size of the effect.

An example of findings reported with p values are below:

Statement: Drug 23 reduced patients' symptoms compared to Drug 22. Patients who received Drug 23 (n=100) were 2.1 times less likely than patients who received Drug 22 (n = 100) to experience symptoms of Disease A, p<0.05.

Statement:Individuals who were prescribed Drug 23 experienced fewer symptoms (M = 1.3, SD = 0.7) compared to individuals who were prescribed Drug 22 (M = 5.3, SD = 1.9). This finding was statistically significant, p= 0.02.

For either statement, if the threshold had been set at 0.05, the null hypothesis (that there was no relationship) should be rejected, and we should conclude significant differences. Noticeably, as can be seen in the two statements above, some researchers will report findings with < or > and others will provide an exact p-value (0.000001) but never zero [6] . When examining research, readers should understand how p values are reported. The best practice is to report all p values for all variables within a study design, rather than only providing p values for variables with significant findings. [7]  The inclusion of all p values provides evidence for study validity and limits suspicion for selective reporting/data mining.  

While researchers have historically used p values, experts who find p values problematic encourage the use of confidence intervals. [8] . P-values alone do not allow us to understand the size or the extent of the differences or associations. [3]  In March 2016, the American Statistical Association (ASA) released a statement on p values, noting that scientific decision-making and conclusions should not be based on a fixed p-value threshold (e.g., 0.05). They recommend focusing on the significance of results in the context of study design, quality of measurements, and validity of data. Ultimately, the ASA statement noted that in isolation, a p-value does not provide strong evidence. [9]

When conceptualizing clinical work, healthcare professionals should consider p values with a concurrent appraisal study design validity. For example, a p-value from a double-blinded randomized clinical trial (designed to minimize bias) should be weighted higher than one from a retrospective observational study [7] . The p-value debate has smoldered since the 1950s [10] , and replacement with confidence intervals has been suggested since the 1980s. [11]

Confidence Intervals

A confidence interval provides a range of values within given confidence (e.g., 95%), including the accurate value of the statistical constraint within a targeted population. [12]  Most research uses a 95% CI, but investigators can set any level (e.g., 90% CI, 99% CI). [13]  A CI provides a range with the lower bound and upper bound limits of a difference or association that would be plausible for a population. [14]  Therefore, a CI of 95% indicates that if a study were to be carried out 100 times, the range would contain the true value in 95, [15]  confidence intervals provide more evidence regarding the precision of an estimate compared to p-values. [6]

In consideration of the similar research example provided above, one could make the following statement with 95% CI:

Statement: Individuals who were prescribed Drug 23 had no symptoms after three days, which was significantly faster than those prescribed Drug 22; there was a mean difference between the two groups of days to the recovery of 4.2 days (95% CI: 1.9 – 7.8).

It is important to note that the width of the CI is affected by the standard error and the sample size; reducing a study sample number will result in less precision of the CI (increase the width). [14]  A larger width indicates a smaller sample size or a larger variability. [16]  A researcher would want to increase the precision of the CI. For example, a 95% CI of 1.43 – 1.47 is much more precise than the one provided in the example above. In research and clinical practice, CIs provide valuable information on whether the interval includes or excludes any clinically significant values. [14]

Null values are sometimes used for differences with CI (zero for differential comparisons and 1 for ratios). However, CIs provide more information than that. [15]  Consider this example: A hospital implements a new protocol that reduced wait time for patients in the emergency department by an average of 25 minutes (95% CI: -2.5 – 41 minutes). Because the range crosses zero, implementing this protocol in different populations could result in longer wait times; however, the range is much higher on the positive side. Thus, while the p-value used to detect statistical significance for this may result in "not significant" findings, individuals should examine this range, consider the study design, and weigh whether or not it is still worth piloting in their workplace.

Similarly to p-values, 95% CIs cannot control for researchers' errors (e.g., study bias or improper data analysis). [14]  In consideration of whether to report p-values or CIs, researchers should examine journal preferences. When in doubt, reporting both may be beneficial. [13]  An example is below:

Reporting both: Individuals who were prescribed Drug 23 had no symptoms after three days, which was significantly faster than those prescribed Drug 22, p = 0.009. There was a mean difference between the two groups of days to the recovery of 4.2 days (95% CI: 1.9 – 7.8).

• Clinical Significance

Recall that clinical significance and statistical significance are two different concepts. Healthcare providers should remember that a study with statistically significant differences and large sample size may be of no interest to clinicians, whereas a study with smaller sample size and statistically non-significant results could impact clinical practice. [14]  Additionally, as previously mentioned, a non-significant finding may reflect the study design itself rather than relationships between variables.

Healthcare providers using evidence-based medicine to inform practice should use clinical judgment to determine the practical importance of studies through careful evaluation of the design, sample size, power, likelihood of type I and type II errors, data analysis, and reporting of statistical findings (p values, 95% CI or both). [4]  Interestingly, some experts have called for "statistically significant" or "not significant" to be excluded from work as statistical significance never has and will never be equivalent to clinical significance. [17]

The decision on what is clinically significant can be challenging, depending on the providers' experience and especially the severity of the disease. Providers should use their knowledge and experiences to determine the meaningfulness of study results and make inferences based not only on significant or insignificant results by researchers but through their understanding of study limitations and practical implications.

• Nursing, Allied Health, and Interprofessional Team Interventions

All physicians, nurses, pharmacists, and other healthcare professionals should strive to understand the concepts in this chapter. These individuals should maintain the ability to review and incorporate new literature for evidence-based and safe care. 

• Review Questions
• Access free multiple choice questions on this topic.
• Comment on this article.

Disclosure: Jacob Shreffler declares no relevant financial relationships with ineligible companies.

Disclosure: Martin Huecker declares no relevant financial relationships with ineligible companies.

This book is distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0) ( http://creativecommons.org/licenses/by-nc-nd/4.0/ ), which permits others to distribute the work, provided that the article is not altered or used commercially. You are not required to obtain permission to distribute this article, provided that you credit the author and journal.

• Cite this Page Shreffler J, Huecker MR. Hypothesis Testing, P Values, Confidence Intervals, and Significance. [Updated 2023 Mar 13]. In: StatPearls [Internet]. Treasure Island (FL): StatPearls Publishing; 2024 Jan-.

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Hypothesis Testing

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A hypothesis test is a statistical inference method used to test the significance of a proposed (hypothesized) relation between population statistics (parameters) and their corresponding sample estimators . In other words, hypothesis tests are used to determine if there is enough evidence in a sample to prove a hypothesis true for the entire population.

The test considers two hypotheses: the null hypothesis , which is a statement meant to be tested, usually something like "there is no effect" with the intention of proving this false, and the alternate hypothesis , which is the statement meant to stand after the test is performed. The two hypotheses must be mutually exclusive ; moreover, in most applications, the two are complementary (one being the negation of the other). The test works by comparing the \(p\)-value to the level of significance (a chosen target). If the \(p\)-value is less than or equal to the level of significance, then the null hypothesis is rejected.

When analyzing data, only samples of a certain size might be manageable as efficient computations. In some situations the error terms follow a continuous or infinite distribution, hence the use of samples to suggest accuracy of the chosen test statistics. The method of hypothesis testing gives an advantage over guessing what distribution or which parameters the data follows.

Definitions and Methodology

Hypothesis test and confidence intervals.

In statistical inference, properties (parameters) of a population are analyzed by sampling data sets. Given assumptions on the distribution, i.e. a statistical model of the data, certain hypotheses can be deduced from the known behavior of the model. These hypotheses must be tested against sampled data from the population.

The null hypothesis \((\)denoted \(H_0)\) is a statement that is assumed to be true. If the null hypothesis is rejected, then there is enough evidence (statistical significance) to accept the alternate hypothesis \((\)denoted \(H_1).\) Before doing any test for significance, both hypotheses must be clearly stated and non-conflictive, i.e. mutually exclusive, statements. Rejecting the null hypothesis, given that it is true, is called a type I error and it is denoted \(\alpha\), which is also its probability of occurrence. Failing to reject the null hypothesis, given that it is false, is called a type II error and it is denoted \(\beta\), which is also its probability of occurrence. Also, \(\alpha\) is known as the significance level , and \(1-\beta\) is known as the power of the test. \(H_0\) \(\textbf{is true}\)\(\hspace{15mm}\) \(H_0\) \(\textbf{is false}\) \(\textbf{Reject}\) \(H_0\)\(\hspace{10mm}\) Type I error Correct Decision \(\textbf{Reject}\) \(H_1\) Correct Decision Type II error The test statistic is the standardized value following the sampled data under the assumption that the null hypothesis is true, and a chosen particular test. These tests depend on the statistic to be studied and the assumed distribution it follows, e.g. the population mean following a normal distribution. The \(p\)-value is the probability of observing an extreme test statistic in the direction of the alternate hypothesis, given that the null hypothesis is true. The critical value is the value of the assumed distribution of the test statistic such that the probability of making a type I error is small.

Methodologies: Given an estimator \(\hat \theta\) of a population statistic \(\theta\), following a probability distribution \(P(T)\), computed from a sample \(\mathcal{S},\) and given a significance level \(\alpha\) and test statistic \(t^*,\) define \(H_0\) and \(H_1;\) compute the test statistic \(t^*.\) \(p\)-value Approach (most prevalent): Find the \(p\)-value using \(t^*\) (right-tailed). If the \(p\)-value is at most \(\alpha,\) reject \(H_0\). Otherwise, reject \(H_1\). Critical Value Approach: Find the critical value solving the equation \(P(T\geq t_\alpha)=\alpha\) (right-tailed). If \(t^*>t_\alpha\), reject \(H_0\). Otherwise, reject \(H_1\). Note: Failing to reject \(H_0\) only means inability to accept \(H_1\), and it does not mean to accept \(H_0\).

Assume a normally distributed population has recorded cholesterol levels with various statistics computed. From a sample of 100 subjects in the population, the sample mean was 214.12 mg/dL (milligrams per deciliter), with a sample standard deviation of 45.71 mg/dL. Perform a hypothesis test, with significance level 0.05, to test if there is enough evidence to conclude that the population mean is larger than 200 mg/dL. Hypothesis Test We will perform a hypothesis test using the \(p\)-value approach with significance level \(\alpha=0.05:\) Define \(H_0\): \(\mu=200\). Define \(H_1\): \(\mu>200\). Since our values are normally distributed, the test statistic is \(z^*=\frac{\bar X - \mu_0}{\frac{s}{\sqrt{n}}}=\frac{214.12 - 200}{\frac{45.71}{\sqrt{100}}}\approx 3.09\). Using a standard normal distribution, we find that our \(p\)-value is approximately \(0.001\). Since the \(p\)-value is at most \(\alpha=0.05,\) we reject \(H_0\). Therefore, we can conclude that the test shows sufficient evidence to support the claim that \(\mu\) is larger than \(200\) mg/dL.

If the sample size was smaller, the normal and \(t\)-distributions behave differently. Also, the question itself must be managed by a double-tail test instead.

Assume a population's cholesterol levels are recorded and various statistics are computed. From a sample of 25 subjects, the sample mean was 214.12 mg/dL (milligrams per deciliter), with a sample standard deviation of 45.71 mg/dL. Perform a hypothesis test, with significance level 0.05, to test if there is enough evidence to conclude that the population mean is not equal to 200 mg/dL. Hypothesis Test We will perform a hypothesis test using the \(p\)-value approach with significance level \(\alpha=0.05\) and the \(t\)-distribution with 24 degrees of freedom: Define \(H_0\): \(\mu=200\). Define \(H_1\): \(\mu\neq 200\). Using the \(t\)-distribution, the test statistic is \(t^*=\frac{\bar X - \mu_0}{\frac{s}{\sqrt{n}}}=\frac{214.12 - 200}{\frac{45.71}{\sqrt{25}}}\approx 1.54\). Using a \(t\)-distribution with 24 degrees of freedom, we find that our \(p\)-value is approximately \(2(0.068)=0.136\). We have multiplied by two since this is a two-tailed argument, i.e. the mean can be smaller than or larger than. Since the \(p\)-value is larger than \(\alpha=0.05,\) we fail to reject \(H_0\). Therefore, the test does not show sufficient evidence to support the claim that \(\mu\) is not equal to \(200\) mg/dL.

The complement of the rejection on a two-tailed hypothesis test (with significance level \(\alpha\)) for a population parameter \(\theta\) is equivalent to finding a confidence interval \((\)with confidence level \(1-\alpha)\) for the population parameter \(\theta\). If the assumption on the parameter \(\theta\) falls inside the confidence interval, then the test has failed to reject the null hypothesis \((\)with \(p\)-value greater than \(\alpha).\) Otherwise, if \(\theta\) does not fall in the confidence interval, then the null hypothesis is rejected in favor of the alternate \((\)with \(p\)-value at most \(\alpha).\)

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What Is Hypothesis Testing?

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Hypothesis Testing: 4 Steps and Example

Hypothesis testing, sometimes called significance testing, is an act in statistics whereby an analyst tests an assumption regarding a population parameter. The methodology employed by the analyst depends on the nature of the data used and the reason for the analysis.

Hypothesis testing is used to assess the plausibility of a hypothesis by using sample data. Such data may come from a larger population or a data-generating process. The word "population" will be used for both of these cases in the following descriptions.

Key Takeaways

• Hypothesis testing is used to assess the plausibility of a hypothesis by using sample data.
• The test provides evidence concerning the plausibility of the hypothesis, given the data.
• Statistical analysts test a hypothesis by measuring and examining a random sample of the population being analyzed.
• The four steps of hypothesis testing include stating the hypotheses, formulating an analysis plan, analyzing the sample data, and analyzing the result.

How Hypothesis Testing Works

In hypothesis testing, an  analyst  tests a statistical sample, intending to provide evidence on the plausibility of the null hypothesis. Statistical analysts measure and examine a random sample of the population being analyzed. All analysts use a random population sample to test two different hypotheses: the null hypothesis and the alternative hypothesis.

The null hypothesis is usually a hypothesis of equality between population parameters; e.g., a null hypothesis may state that the population mean return is equal to zero. The alternative hypothesis is effectively the opposite of a null hypothesis. Thus, they are mutually exclusive , and only one can be true. However, one of the two hypotheses will always be true.

The null hypothesis is a statement about a population parameter, such as the population mean, that is assumed to be true.

• State the hypotheses.
• Formulate an analysis plan, which outlines how the data will be evaluated.
• Carry out the plan and analyze the sample data.
• Analyze the results and either reject the null hypothesis, or state that the null hypothesis is plausible, given the data.

Example of Hypothesis Testing

If an individual wants to test that a penny has exactly a 50% chance of landing on heads, the null hypothesis would be that 50% is correct, and the alternative hypothesis would be that 50% is not correct. Mathematically, the null hypothesis is represented as Ho: P = 0.5. The alternative hypothesis is shown as "Ha" and is identical to the null hypothesis, except with the equal sign struck-through, meaning that it does not equal 50%.

A random sample of 100 coin flips is taken, and the null hypothesis is tested. If it is found that the 100 coin flips were distributed as 40 heads and 60 tails, the analyst would assume that a penny does not have a 50% chance of landing on heads and would reject the null hypothesis and accept the alternative hypothesis.

If there were 48 heads and 52 tails, then it is plausible that the coin could be fair and still produce such a result. In cases such as this where the null hypothesis is "accepted," the analyst states that the difference between the expected results (50 heads and 50 tails) and the observed results (48 heads and 52 tails) is "explainable by chance alone."

When Did Hypothesis Testing Begin?

Some statisticians attribute the first hypothesis tests to satirical writer John Arbuthnot in 1710, who studied male and female births in England after observing that in nearly every year, male births exceeded female births by a slight proportion. Arbuthnot calculated that the probability of this happening by chance was small, and therefore it was due to “divine providence.”

What are the Benefits of Hypothesis Testing?

Hypothesis testing helps assess the accuracy of new ideas or theories by testing them against data. This allows researchers to determine whether the evidence supports their hypothesis, helping to avoid false claims and conclusions. Hypothesis testing also provides a framework for decision-making based on data rather than personal opinions or biases. By relying on statistical analysis, hypothesis testing helps to reduce the effects of chance and confounding variables, providing a robust framework for making informed conclusions.

What are the Limitations of Hypothesis Testing?

Hypothesis testing relies exclusively on data and doesn’t provide a comprehensive understanding of the subject being studied. Additionally, the accuracy of the results depends on the quality of the available data and the statistical methods used. Inaccurate data or inappropriate hypothesis formulation may lead to incorrect conclusions or failed tests. Hypothesis testing can also lead to errors, such as analysts either accepting or rejecting a null hypothesis when they shouldn’t have. These errors may result in false conclusions or missed opportunities to identify significant patterns or relationships in the data.

Hypothesis testing refers to a statistical process that helps researchers determine the reliability of a study. By using a well-formulated hypothesis and set of statistical tests, individuals or businesses can make inferences about the population that they are studying and draw conclusions based on the data presented. All hypothesis testing methods have the same four-step process, which includes stating the hypotheses, formulating an analysis plan, analyzing the sample data, and analyzing the result.

Sage. " Introduction to Hypothesis Testing ," Page 4.

Elder Research. " Who Invented the Null Hypothesis? "

Formplus. " Hypothesis Testing: Definition, Uses, Limitations and Examples ."

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Understanding Hypothesis Testing

Hypothesis testing involves formulating assumptions about population parameters based on sample statistics and rigorously evaluating these assumptions against empirical evidence. This article sheds light on the significance of hypothesis testing and the critical steps involved in the process.

What is Hypothesis Testing?

A hypothesis is an assumption or idea, specifically a statistical claim about an unknown population parameter. For example, a judge assumes a person is innocent and verifies this by reviewing evidence and hearing testimony before reaching a verdict.

Hypothesis testing is a statistical method that is used to make a statistical decision using experimental data. Hypothesis testing is basically an assumption that we make about a population parameter. It evaluates two mutually exclusive statements about a population to determine which statement is best supported by the sample data. 

To test the validity of the claim or assumption about the population parameter:

• A sample is drawn from the population and analyzed.
• The results of the analysis are used to decide whether the claim is true or not.

Example: You say an average height in the class is 30 or a boy is taller than a girl. All of these is an assumption that we are assuming, and we need some statistical way to prove these. We need some mathematical conclusion whatever we are assuming is true.

Defining Hypotheses

• Null hypothesis (H 0 ): In statistics, the null hypothesis is a general statement or default position that there is no relationship between two measured cases or no relationship among groups. In other words, it is a basic assumption or made based on the problem knowledge. Example : A company’s mean production is 50 units/per da H 0 : [Tex]\mu [/Tex] = 50.
• Alternative hypothesis (H 1 ): The alternative hypothesis is the hypothesis used in hypothesis testing that is contrary to the null hypothesis.  Example: A company’s production is not equal to 50 units/per day i.e. H 1 : [Tex]\mu [/Tex] [Tex]\ne [/Tex] 50.

Key Terms of Hypothesis Testing

• Level of significance : It refers to the degree of significance in which we accept or reject the null hypothesis. 100% accuracy is not possible for accepting a hypothesis, so we, therefore, select a level of significance that is usually 5%. This is normally denoted with  [Tex]\alpha[/Tex] and generally, it is 0.05 or 5%, which means your output should be 95% confident to give a similar kind of result in each sample.
• P-value: The P value , or calculated probability, is the probability of finding the observed/extreme results when the null hypothesis(H0) of a study-given problem is true. If your P-value is less than the chosen significance level then you reject the null hypothesis i.e. accept that your sample claims to support the alternative hypothesis.
• Test Statistic: The test statistic is a numerical value calculated from sample data during a hypothesis test, used to determine whether to reject the null hypothesis. It is compared to a critical value or p-value to make decisions about the statistical significance of the observed results.
• Critical value : The critical value in statistics is a threshold or cutoff point used to determine whether to reject the null hypothesis in a hypothesis test.
• Degrees of freedom: Degrees of freedom are associated with the variability or freedom one has in estimating a parameter. The degrees of freedom are related to the sample size and determine the shape.

Why do we use Hypothesis Testing?

Hypothesis testing is an important procedure in statistics. Hypothesis testing evaluates two mutually exclusive population statements to determine which statement is most supported by sample data. When we say that the findings are statistically significant, thanks to hypothesis testing. 

One-Tailed and Two-Tailed Test

One tailed test focuses on one direction, either greater than or less than a specified value. We use a one-tailed test when there is a clear directional expectation based on prior knowledge or theory. The critical region is located on only one side of the distribution curve. If the sample falls into this critical region, the null hypothesis is rejected in favor of the alternative hypothesis.

One-Tailed Test

There are two types of one-tailed test:

• Left-Tailed (Left-Sided) Test: The alternative hypothesis asserts that the true parameter value is less than the null hypothesis. Example: H 0 ​: [Tex]\mu \geq 50 [/Tex] and H 1 : [Tex]\mu < 50 [/Tex]
• Right-Tailed (Right-Sided) Test : The alternative hypothesis asserts that the true parameter value is greater than the null hypothesis. Example: H 0 : [Tex]\mu \leq50 [/Tex] and H 1 : [Tex]\mu > 50 [/Tex]

Two-Tailed Test

A two-tailed test considers both directions, greater than and less than a specified value.We use a two-tailed test when there is no specific directional expectation, and want to detect any significant difference.

Example: H 0 : [Tex]\mu = [/Tex] 50 and H 1 : [Tex]\mu \neq 50 [/Tex]

To delve deeper into differences into both types of test: Refer to link

What are Type 1 and Type 2 errors in Hypothesis Testing?

In hypothesis testing, Type I and Type II errors are two possible errors that researchers can make when drawing conclusions about a population based on a sample of data. These errors are associated with the decisions made regarding the null hypothesis and the alternative hypothesis.

• Type I error: When we reject the null hypothesis, although that hypothesis was true. Type I error is denoted by alpha( [Tex]\alpha [/Tex] ).
• Type II errors : When we accept the null hypothesis, but it is false. Type II errors are denoted by beta( [Tex]\beta [/Tex] ).

How does Hypothesis Testing work?

Step 1: define null and alternative hypothesis.

State the null hypothesis ( [Tex]H_0 [/Tex] ), representing no effect, and the alternative hypothesis ( [Tex]H_1 [/Tex] ​), suggesting an effect or difference.

We first identify the problem about which we want to make an assumption keeping in mind that our assumption should be contradictory to one another, assuming Normally distributed data.

Step 2 – Choose significance level

Select a significance level ( [Tex]\alpha [/Tex] ), typically 0.05, to determine the threshold for rejecting the null hypothesis. It provides validity to our hypothesis test, ensuring that we have sufficient data to back up our claims. Usually, we determine our significance level beforehand of the test. The p-value is the criterion used to calculate our significance value.

Step 3 – Collect and Analyze data.

Gather relevant data through observation or experimentation. Analyze the data using appropriate statistical methods to obtain a test statistic.

Step 4-Calculate Test Statistic

The data for the tests are evaluated in this step we look for various scores based on the characteristics of data. The choice of the test statistic depends on the type of hypothesis test being conducted.

There are various hypothesis tests, each appropriate for various goal to calculate our test. This could be a Z-test , Chi-square , T-test , and so on.

• Z-test : If population means and standard deviations are known. Z-statistic is commonly used.
• t-test : If population standard deviations are unknown. and sample size is small than t-test statistic is more appropriate.
• Chi-square test : Chi-square test is used for categorical data or for testing independence in contingency tables
• F-test : F-test is often used in analysis of variance (ANOVA) to compare variances or test the equality of means across multiple groups.

We have a smaller dataset, So, T-test is more appropriate to test our hypothesis.

T-statistic is a measure of the difference between the means of two groups relative to the variability within each group. It is calculated as the difference between the sample means divided by the standard error of the difference. It is also known as the t-value or t-score.

Step 5 – Comparing Test Statistic:

In this stage, we decide where we should accept the null hypothesis or reject the null hypothesis. There are two ways to decide where we should accept or reject the null hypothesis.

Method A: Using Crtical values

Comparing the test statistic and tabulated critical value we have,

• If Test Statistic>Critical Value: Reject the null hypothesis.
• If Test Statistic≤Critical Value: Fail to reject the null hypothesis.

Note: Critical values are predetermined threshold values that are used to make a decision in hypothesis testing. To determine critical values for hypothesis testing, we typically refer to a statistical distribution table , such as the normal distribution or t-distribution tables based on.

Method B: Using P-values

We can also come to an conclusion using the p-value,

• If the p-value is less than or equal to the significance level i.e. ( [Tex]p\leq\alpha [/Tex] ), you reject the null hypothesis. This indicates that the observed results are unlikely to have occurred by chance alone, providing evidence in favor of the alternative hypothesis.
• If the p-value is greater than the significance level i.e. ( [Tex]p\geq \alpha[/Tex] ), you fail to reject the null hypothesis. This suggests that the observed results are consistent with what would be expected under the null hypothesis.

Note : The p-value is the probability of obtaining a test statistic as extreme as, or more extreme than, the one observed in the sample, assuming the null hypothesis is true. To determine p-value for hypothesis testing, we typically refer to a statistical distribution table , such as the normal distribution or t-distribution tables based on.

Step 7- Interpret the Results

At last, we can conclude our experiment using method A or B.

Calculating test statistic

To validate our hypothesis about a population parameter we use statistical functions . We use the z-score, p-value, and level of significance(alpha) to make evidence for our hypothesis for normally distributed data .

1. Z-statistics:

When population means and standard deviations are known.

[Tex]z = \frac{\bar{x} – \mu}{\frac{\sigma}{\sqrt{n}}}[/Tex]

• [Tex]\bar{x} [/Tex] is the sample mean,
• μ represents the population mean, 
• σ is the standard deviation
• and n is the size of the sample.

2. T-Statistics

T test is used when n<30,

t-statistic calculation is given by:

[Tex]t=\frac{x̄-μ}{s/\sqrt{n}} [/Tex]

• t = t-score,
• x̄ = sample mean
• μ = population mean,
• s = standard deviation of the sample,
• n = sample size

3. Chi-Square Test

Chi-Square Test for Independence categorical Data (Non-normally distributed) using:

[Tex]\chi^2 = \sum \frac{(O_{ij} – E_{ij})^2}{E_{ij}}[/Tex]

• [Tex]O_{ij}[/Tex] is the observed frequency in cell [Tex]{ij} [/Tex]
• i,j are the rows and columns index respectively.
• [Tex]E_{ij}[/Tex] is the expected frequency in cell [Tex]{ij}[/Tex] , calculated as : [Tex]\frac{{\text{{Row total}} \times \text{{Column total}}}}{{\text{{Total observations}}}}[/Tex]

Real life Examples of Hypothesis Testing

Let’s examine hypothesis testing using two real life situations,

Case A: D oes a New Drug Affect Blood Pressure?

Imagine a pharmaceutical company has developed a new drug that they believe can effectively lower blood pressure in patients with hypertension. Before bringing the drug to market, they need to conduct a study to assess its impact on blood pressure.

• Before Treatment: 120, 122, 118, 130, 125, 128, 115, 121, 123, 119
• After Treatment: 115, 120, 112, 128, 122, 125, 110, 117, 119, 114

Step 1 : Define the Hypothesis

• Null Hypothesis : (H 0 )The new drug has no effect on blood pressure.
• Alternate Hypothesis : (H 1 )The new drug has an effect on blood pressure.

Step 2: Define the Significance level

Let’s consider the Significance level at 0.05, indicating rejection of the null hypothesis.

If the evidence suggests less than a 5% chance of observing the results due to random variation.

Step 3 : Compute the test statistic

Using paired T-test analyze the data to obtain a test statistic and a p-value.

The test statistic (e.g., T-statistic) is calculated based on the differences between blood pressure measurements before and after treatment.

t = m/(s/√n)

• m  = mean of the difference i.e X after, X before
• s  = standard deviation of the difference (d) i.e d i ​= X after, i ​− X before,
• n  = sample size,

then, m= -3.9, s= 1.8 and n= 10

we, calculate the , T-statistic = -9 based on the formula for paired t test

Step 4: Find the p-value

The calculated t-statistic is -9 and degrees of freedom df = 9, you can find the p-value using statistical software or a t-distribution table.

thus, p-value = 8.538051223166285e-06

Step 5: Result

• If the p-value is less than or equal to 0.05, the researchers reject the null hypothesis.
• If the p-value is greater than 0.05, they fail to reject the null hypothesis.

Conclusion: Since the p-value (8.538051223166285e-06) is less than the significance level (0.05), the researchers reject the null hypothesis. There is statistically significant evidence that the average blood pressure before and after treatment with the new drug is different.

Python Implementation of Case A

Let’s create hypothesis testing with python, where we are testing whether a new drug affects blood pressure. For this example, we will use a paired T-test. We’ll use the scipy.stats library for the T-test.

Scipy is a mathematical library in Python that is mostly used for mathematical equations and computations.

We will implement our first real life problem via python,

import numpy as np from scipy import stats # Data before_treatment = np . array ([ 120 , 122 , 118 , 130 , 125 , 128 , 115 , 121 , 123 , 119 ]) after_treatment = np . array ([ 115 , 120 , 112 , 128 , 122 , 125 , 110 , 117 , 119 , 114 ]) # Step 1: Null and Alternate Hypotheses # Null Hypothesis: The new drug has no effect on blood pressure. # Alternate Hypothesis: The new drug has an effect on blood pressure. null_hypothesis = "The new drug has no effect on blood pressure." alternate_hypothesis = "The new drug has an effect on blood pressure." # Step 2: Significance Level alpha = 0.05 # Step 3: Paired T-test t_statistic , p_value = stats . ttest_rel ( after_treatment , before_treatment ) # Step 4: Calculate T-statistic manually m = np . mean ( after_treatment - before_treatment ) s = np . std ( after_treatment - before_treatment , ddof = 1 ) # using ddof=1 for sample standard deviation n = len ( before_treatment ) t_statistic_manual = m / ( s / np . sqrt ( n )) # Step 5: Decision if p_value <= alpha : decision = "Reject" else : decision = "Fail to reject" # Conclusion if decision == "Reject" : conclusion = "There is statistically significant evidence that the average blood pressure before and after treatment with the new drug is different." else : conclusion = "There is insufficient evidence to claim a significant difference in average blood pressure before and after treatment with the new drug." # Display results print ( "T-statistic (from scipy):" , t_statistic ) print ( "P-value (from scipy):" , p_value ) print ( "T-statistic (calculated manually):" , t_statistic_manual ) print ( f "Decision: { decision } the null hypothesis at alpha= { alpha } ." ) print ( "Conclusion:" , conclusion )

T-statistic (from scipy): -9.0 P-value (from scipy): 8.538051223166285e-06 T-statistic (calculated manually): -9.0 Decision: Reject the null hypothesis at alpha=0.05. Conclusion: There is statistically significant evidence that the average blood pressure before and after treatment with the new drug is different.

In the above example, given the T-statistic of approximately -9 and an extremely small p-value, the results indicate a strong case to reject the null hypothesis at a significance level of 0.05. 

• The results suggest that the new drug, treatment, or intervention has a significant effect on lowering blood pressure.
• The negative T-statistic indicates that the mean blood pressure after treatment is significantly lower than the assumed population mean before treatment.

Case B : Cholesterol level in a population

Data: A sample of 25 individuals is taken, and their cholesterol levels are measured.

Cholesterol Levels (mg/dL): 205, 198, 210, 190, 215, 205, 200, 192, 198, 205, 198, 202, 208, 200, 205, 198, 205, 210, 192, 205, 198, 205, 210, 192, 205.

Populations Mean = 200

Population Standard Deviation (σ): 5 mg/dL(given for this problem)

Step 1: Define the Hypothesis

• Null Hypothesis (H 0 ): The average cholesterol level in a population is 200 mg/dL.
• Alternate Hypothesis (H 1 ): The average cholesterol level in a population is different from 200 mg/dL.

As the direction of deviation is not given , we assume a two-tailed test, and based on a normal distribution table, the critical values for a significance level of 0.05 (two-tailed) can be calculated through the z-table and are approximately -1.96 and 1.96.

The test statistic is calculated by using the z formula Z = [Tex](203.8 – 200) / (5 \div \sqrt{25}) [/Tex] ​ and we get accordingly , Z =2.039999999999992.

Step 4: Result

Since the absolute value of the test statistic (2.04) is greater than the critical value (1.96), we reject the null hypothesis. And conclude that, there is statistically significant evidence that the average cholesterol level in the population is different from 200 mg/dL

Python Implementation of Case B

import scipy.stats as stats import math import numpy as np # Given data sample_data = np . array ( [ 205 , 198 , 210 , 190 , 215 , 205 , 200 , 192 , 198 , 205 , 198 , 202 , 208 , 200 , 205 , 198 , 205 , 210 , 192 , 205 , 198 , 205 , 210 , 192 , 205 ]) population_std_dev = 5 population_mean = 200 sample_size = len ( sample_data ) # Step 1: Define the Hypotheses # Null Hypothesis (H0): The average cholesterol level in a population is 200 mg/dL. # Alternate Hypothesis (H1): The average cholesterol level in a population is different from 200 mg/dL. # Step 2: Define the Significance Level alpha = 0.05 # Two-tailed test # Critical values for a significance level of 0.05 (two-tailed) critical_value_left = stats . norm . ppf ( alpha / 2 ) critical_value_right = - critical_value_left # Step 3: Compute the test statistic sample_mean = sample_data . mean () z_score = ( sample_mean - population_mean ) / \ ( population_std_dev / math . sqrt ( sample_size )) # Step 4: Result # Check if the absolute value of the test statistic is greater than the critical values if abs ( z_score ) > max ( abs ( critical_value_left ), abs ( critical_value_right )): print ( "Reject the null hypothesis." ) print ( "There is statistically significant evidence that the average cholesterol level in the population is different from 200 mg/dL." ) else : print ( "Fail to reject the null hypothesis." ) print ( "There is not enough evidence to conclude that the average cholesterol level in the population is different from 200 mg/dL." )

Reject the null hypothesis. There is statistically significant evidence that the average cholesterol level in the population is different from 200 mg/dL.

Limitations of Hypothesis Testing

• Although a useful technique, hypothesis testing does not offer a comprehensive grasp of the topic being studied. Without fully reflecting the intricacy or whole context of the phenomena, it concentrates on certain hypotheses and statistical significance.
• The accuracy of hypothesis testing results is contingent on the quality of available data and the appropriateness of statistical methods used. Inaccurate data or poorly formulated hypotheses can lead to incorrect conclusions.
• Relying solely on hypothesis testing may cause analysts to overlook significant patterns or relationships in the data that are not captured by the specific hypotheses being tested. This limitation underscores the importance of complimenting hypothesis testing with other analytical approaches.

Hypothesis testing stands as a cornerstone in statistical analysis, enabling data scientists to navigate uncertainties and draw credible inferences from sample data. By systematically defining null and alternative hypotheses, choosing significance levels, and leveraging statistical tests, researchers can assess the validity of their assumptions. The article also elucidates the critical distinction between Type I and Type II errors, providing a comprehensive understanding of the nuanced decision-making process inherent in hypothesis testing. The real-life example of testing a new drug’s effect on blood pressure using a paired T-test showcases the practical application of these principles, underscoring the importance of statistical rigor in data-driven decision-making.

Frequently Asked Questions (FAQs)

1. what are the 3 types of hypothesis test.

There are three types of hypothesis tests: right-tailed, left-tailed, and two-tailed. Right-tailed tests assess if a parameter is greater, left-tailed if lesser. Two-tailed tests check for non-directional differences, greater or lesser.

2.What are the 4 components of hypothesis testing?

Null Hypothesis ( [Tex]H_o [/Tex] ): No effect or difference exists. Alternative Hypothesis ( [Tex]H_1 [/Tex] ): An effect or difference exists. Significance Level ( [Tex]\alpha [/Tex] ): Risk of rejecting null hypothesis when it’s true (Type I error). Test Statistic: Numerical value representing observed evidence against null hypothesis.

3.What is hypothesis testing in ML?

Statistical method to evaluate the performance and validity of machine learning models. Tests specific hypotheses about model behavior, like whether features influence predictions or if a model generalizes well to unseen data.

4.What is the difference between Pytest and hypothesis in Python?

Pytest purposes general testing framework for Python code while Hypothesis is a Property-based testing framework for Python, focusing on generating test cases based on specified properties of the code.

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